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Genome wide rhythmic transcription under light/dark cycles in Ostreococcus tauri

ABSTRACT: Genome wide rhythmic transcription under light/dark cycles is associated with sequential transcription of specific biological processes genes in Ostreococcus tauri. Transcriptional profiling of Ostreococcus tauri under light/dark cycles. In order to identify genes with a diurnal rhythm, cells entrained under 12:12 light/dark cycles were sampled every 3 hours for 27 hours with two overlapping time points at Time 9 (Light ON at Time 9; Light OFF at Time 21) in 3 independent experiments.

ORGANISM(S): Ostreococcus tauri

PROVIDER: GSE16422 | GEO | 2010/04/20

SECONDARY ACCESSION(S): PRJNA116365

REPOSITORIES: GEO

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Genome wide rhythmic transcription under light/dark cycles in Ostreococcus tauri

Project description:Genome wide rhythmic transcription under light/dark cycles is associated with sequential transcription of specific biological processes genes in Ostreococcus tauri. Transcriptional profiling of Ostreococcus tauri under light/dark cycles. In order to identify genes with a diurnal rhythm, cells entrained under 12:12 light/dark cycles were sampled every 3 hours for 27 hours with two overlapping time points at Time 9 (Light ON at Time 9; Light OFF at Time 21) in 3 independent experiments. Two-conditions experiment, test situation (kinetic point) vs. Pool of each samples. Biological triplicates: 3 independant kinetics, 9 points per kinetics, Technical triplicates per array.

2010-04-20 | E-GEOD-16422 | biostudies-arrayexpress

Circadian rhythms study of the unicellular algae Ostreococcus tauri by shotgun proteomics: Dark adaptation, Global proteomics.

Project description:The overall set of experiments consist of sampling a culture of the unicellular algae Ostreococcus tauri (wild type) every 24 hours for 4 time points(ZT24, ZT48, ZT72 and ZT96). Each time point was performed with 5 replicate culture flasks, the cultures were grown under dark conditions. This proteome is associated to the transition from an 12:12 light:dark to a dark:dark regime.

2022-12-01 | PXD002909 | Pride

Circadian rhythms study of the unicellular algae Ostreococcus tauri by shotgun proteomics

Project description:The overall set of experiments consist of sampling a culture of the unicellular algae Ostreococcus tauri (wild type) every 4 hours over a period of 24h (6 time points). Each time point was performed with 5 replicate culture flasks, the cultures were grown under a 12:12 hour cycle (light:dark).

2022-12-01 | PXD001734 | Pride

Circadian rhythms study of the unicellular algae Ostreococcus tauri by shotgun proteomics

2022-12-01 | PXD001735 | Pride

Ostreococcus tauri

Project description:Genome wide rhythmic transcription under light/dark cycles in Ostreococcus tauri

| PRJNA116365 | ENA

Circadian rhythms study of the unicellular algae Ostreococcus tauri by shotgun proteomics, sampling under dark condition.

2022-12-01 | PXD002874 | Pride

Circadian rhythms study of the unicellular algae Ostreococcus tauri by shotgun proteomics, sampling under dark condition.

2022-12-01 | PXD002906 | Pride

Unveiling Seasonal Adaptive Molecular Mechanisms in Marine Phytoplankton during Diel Cycles through Multiomics Integration

Project description:Earth tilted rotation and translation around the Sun produce one of the most pervasive rhythms on our planet, giving rise to seasonal variations in diel cycles. Although marine phytoplankton plays a key role on ecosystems, multiomics analysis of its response to these periodic environmental signals remains largely unexplored. The marine picoalga Ostreococcus tauri, which resides at the base of the green lineage, has been chosen as model organism due to its high abundance in marine phytoplankton and its cellular and genomic simplicity, making it the smallest free living eukaryote. Ostreococcus was subjected to different light regimes: summer long days, winter short days, constant light and constant dark conditions, to investigate these responses. Although 80% of the transcriptome presented diel rhythmicity, less than 5% maintained oscillations under constant conditions. A drastic reduction in proteome rhythmicity was observed with 55% of the proteins oscillating. Seasonal specific rhythms were found for key physiological processes such as cell cycle, photosynthesis, carotenoid biosynthesis, starch accumulation and nitrate assimilation. In this study, a global orchestration between transcriptome, proteome and physiological dynamics was characterised identifying specific seasonal temporal offsets between peaks in transcripts, proteins, and physiological responses.

2023-11-17 | PXD046992 | Pride

Synechococcus elongatus PCC7942 proteome's cyclic rhythm by LC-MSMS

Project description:To uncover the variation in protein abundances during 48 hours under light and dark cycles (12:12-hours), we used quantitative proteomics, with TMT 6-plex isobaric labeling. We queried the S. elongatus proteome at ten different time points spanning a single 24 hour period, leading to 20 time points over the full 48 hour period.

2014-04-02 | PXD000510 | Pride

Transcription profiling of poplar plants grown in different light and temperature cycles

Project description:Poplar (Populus trichocarpa, clone Nisqually-1) plants were grown in a Conviron PGR 15 growth chamber using precise control of temperature, light, and humidity. Diurnal (driven) conditions included 12L:12D light cycles and 25C/12C thermocycles in three different combinations. These were: photocycles (LDHH), 12 hrs. light (L)/12 hrs. dark (D) at a constant temperature (25C; HH); photo/thermocycles (LDHC): 12 hrs. light (L) /12 hrs. dark (D) with a high day temperature (25C) and a low night temperature (12C); and thermocycles (LLHC): continuous light (LL) with 12 hrs. high/12 hrs. low temperature (25C, day; 12C, night). Light intensity and relative humidity were 700 micromol m-2s-2 and 50%, respectively. Three-month-old poplar plants were entrained for at least one week under the respective condition prior to initiation of each experiment. Leaves and stems from individual poplar plants were collected every four hours for 48 hrs in driven (diurnal) conditions followed by a two day freerun spacer under continuous light/temperature followed by two additional days of sampling under the same continuous free run condition.

2010-01-09 | E-MEXP-2509 | biostudies-arrayexpress

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