Project description:In order to study changes in gene expression during mushroom development in Schizophyllum commune, genome wide gene expression was analysed in 4 developmental stages: vegetative mycelium, stage I aggregates, stage II primordia and mature mushrooms
Project description:In order to study changes in gene expression during mushroom development in Schizophyllum commune, genome wide gene expression was analysed in 4 developmental stages: vegetative mycelium, stage I aggregates, stage II primordia and mature mushrooms. In order to study the effects of absence of expression of sc3 and sc4, genome-wide expression was analysed in a dikaryon in which those genes have been inactivated by targeted gene deletion.
Project description:In order to study changes in gene expression during mushroom development in Schizophyllum commune, genome wide gene expression was analysed in 4 developmental stages: vegetative mycelium, stage I aggregates, stage II primordia and mature mushrooms 4 samples: - vegetative mycelium - stage I aggregates - stage II primordia - mature mushrooms RNA was obtained from 3 biological replicates and pooled
Project description:In order to study changes in gene expression during mushroom development in Schizophyllum commune, genome wide gene expression was analysed in 4 developmental stages: vegetative mycelium, stage I aggregates, stage II primordia and mature mushrooms. In order to study the effects of absence of expression of sc3 and sc4, genome-wide expression was analysed in a dikaryon in which those genes have been inactivated by targeted gene deletion. 5 samples: - vegetative mycelium - stage I aggregates - stage II primordia - mature mushrooms - deltaSC3 deltaSC4 dikaryon RNA was obtained from 3 biological replicates and pooled
Project description:Using RNA-Seq data from the mycelium of Fusarium graminearum, we found weakly expressed regions on all of the four chromosomes that exactly matched with non-conserved regions. Comparison of gene expression between two different developmental stages (spores and mycelium) showed that the expression of genes in conserved regions is stable, while gene expression in non-conserved regions is much more influenced by developmental stage.
Project description:Sclerotial formation is one of the most important phases during the morel life cycle, and previous reports indicated that reactive oxygen species (ROS) play an important role in sclerotial formation. However, the mechanisms of ROS response in sclerotial initiation (SI) stage are poorly understood.
Project description:Using RNA-Seq data from the mycelium of Fusarium graminearum, we found weakly expressed regions on all of the four chromosomes that exactly matched with non-conserved regions. Comparison of gene expression between two different developmental stages (spores and mycelium) showed that the expression of genes in conserved regions is stable, while gene expression in non-conserved regions is much more influenced by developmental stage. Examination of gene expression in two developmental stages (spores and mycelium). Three biological replicates were performed for each stage.
Project description:In order to understand the role of phloems of apple dwarfing rootstocks,and investigated the expression differences of dwarfing and vigorous apple stocks in the bud break stage, The phloem tissue at bud break stage(0 DABB(days after buds break) of three apple rootstocks including A1d（a partial GA insensitive mutant of Malus hupehensis ),WT Malus hupehensis and were QZ1(a hybrid of Malus hupensis and a Cylindrical apple variety) were sampled and underwent RNA-Seq analysis.
Project description:The CD20-homolog Ms4a8a was identified as a novel molecule of tumor-associated macrophages directly enhancing tumor growth. In addition, Ms4a8a is expressed in typical M2-dominated pathologies as late stage Trypanosoma congolense and Taenia crassiceps infections. For its induction a complex stimulation with pro- and antiinflammatory mediators is necessary. In this study we analysed how forced over-expression of Ms4a8a modulates the TLR4 response of RAW264.7 macrophages. We used microarrays to assess gene regulatory functions of Ms4a8a in Raw264.7 macrophages
