Transcriptomics

Dataset Information

31

Expression data from side population subfraction hematopoietic stem cells


ABSTRACT: The traditional view of hematopoiesis has been that all the cells of the peripheral blood are the progeny of a unitary homogeneous pool of hematopoietic stem cells (HSCs). Recent evidence suggests that the hematopoietic system is actually maintained by a consortium of HSC subtypes with distinct functional characteristics. We show here that myeloid-biased HSCs (My-HSCs) and lymphoid-biased (Ly-HSCs) can be purified according to their capacity for Hoechst dye efflux in combination with canonical HSC markers. We used microarray expression profiling to determine the transcriptional profiles of myeloid-biased lower-SP HSCs and lymphoid-biased upper-SP HSCs Overall design: Three biological replicates were analyzed for each HSC subpopulation. Lower-SP and upper-SP HSCs were purified from three pools of mice on separate days. HSCs were further purified with the addition of canonical HSC makers; Sca-1+ c-Kit+ Lineage-

REANALYSED by: GSE119128

INSTRUMENT(S): [Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array

ORGANISM(S): Mus musculus  

SUBMITTER: Grant Anthony Challen   

PROVIDER: GSE16475 | GEO | 2010-03-01

SECONDARY ACCESSION(S): PRJNA116315

REPOSITORIES: GEO

altmetric image

Publications

Sorry, this publication's infomation has not been loaded in the Indexer, please go directly to PUBMED or Altmetric.

Similar Datasets

2014-05-01 | E-GEOD-16475 | ArrayExpress
2014-06-03 | E-GEOD-45561 | ArrayExpress
| GSE31574 | GEO
2012-03-27 | E-SMDB-3450 | ArrayExpress
2018-11-28 | PXD011537 | Pride
2015-04-30 | E-GEOD-4332 | ArrayExpress
2014-07-03 | E-GEOD-4332 | ExpressionAtlas
| GSE86209 | GEO
2014-12-10 | E-GEOD-50775 | ArrayExpress
| GSE85745 | GEO