Project description:Analysis of miRNA expression in colon biopsies from Crohn's disease (CD), ulcerative colitis (UC), and non-inflammatory bowel disease (IBD) control subjects.
Project description:Lysine acetylation is a rich post-translational modification in prokaryotes that regulates multiple immune response pathways, and its importance has been overlooked. Crohn's disease is a subtype of inflammatory bowel disease characterized by chronic inflammation involving the entire gastrointestinal tract. CD-induced intestinal damage and subsequent disability is a growing global health problem. However, the pathophysiology of this disease is not fully understood, and the level of protein acetylation in Crohn's gut tissue is not well understood.To determine the level of protein acetylation in different intestinal sites of CD and changes in intestinal immune response after colonic protein acetylation. Colon, cecum, and rectal tissues of Crohn's disease and healthy control mice were detected by acetylation proteomics. ResultsThe analysis determined that there were up to 624 acetylated proteins and 1809 acetylation sites in intestinal tissue, which were different in the disease and control groups, and the acetylated proteins were enriched in immunomodulatory signaling pathways such as NF-κB, MAPK, and Wnt.
Project description:The entire small intestine was observed by balloon endoscopy. Biopsy specimens were taken from jejunum, ileum and colon, respectively. Four Crohn's disease patients and four healthy controls were compared.
Project description:In Crohn's disease, creeping fat is the characteristic expansion of mesenteric adipose tissue wrapping around the inflamed intestine. Through a comparative transcriptomic analysis of creeping fat and normal-looking mesenteric adipose tissues from patients with Crohn's disease and non-Crohn's disease, we found that a dynamic transcriptional and cell compositional change occurs during the progression from non-Crohn's disease to Crohn's disease, and finally to creeping fat.
Project description:This study investigates the presence of specific fibrosis-associated gene expression signatures in Crohn's disease patient biopsies.
Project description:This study evaluated lamina propria phagocyte gene expression and the associated microbiome in samples from Crohn's disease patients and controls.
