Transcriptomics,Genomics

Dataset Information

31

Gene expression analyses of ovarian clear cell cancer cell lines


ABSTRACT: A variety of human cancers demonstrate alterations in microRNA expression. We hypothesized that regulatory defects in microRNAs play a central early role in organizing the molecular changes involved in ovarian cancer (OvCa). Using both gene arrays and deep sequencing, we comprehensively profiled mRNA and microRNA expression, respectively, in human clear cell epithelial OvCa cell lines and short-term primary cultures of normal ovarian surface epithelium. We expected that over-expression of a specific microRNA would lead to lower expression of its mRNA targets, and under-expression of a specific microRNA would lead to higher expression of its target genes. Using our expression data in conjunction with established in silico algorithms, we found putative microRNA:mRNA functional pairs. Keywords: two group comparison; gene expression profiling Overall design: We generated gene expression profiles of a panel of ten OvCa clear cell cell lines, along with six independent normal ovarian surface epithelium (NOSE) cultures.

INSTRUMENT(S): Illumina HumanHT-12 V3.0 expression beadchip

SUBMITTER: Chad Creighton  

PROVIDER: GSE16570 | GEO | 2010-01-28

SECONDARY ACCESSION(S): PRJNA122757

REPOSITORIES: GEO

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MicroRNAs (miRNAs) are small noncoding RNAs that direct gene regulation through translational repression and degradation of complementary mRNA. Although miRNAs have been implicated as oncogenes and tumor suppressors in a variety of human cancers, functional roles for individual miRNAs have not been described in clear cell ovarian carcinoma, an aggressive and chemoresistant subtype of ovarian cancer. We performed deep sequencing to comprehensively profile miRNA expression in 10 human clear cell o  ...[more]

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