Transcriptomics,Genomics

Dataset Information

18

Gene expression analyses of mir-31 overexpression in ovarian serous cancer cell line


ABSTRACT: A variety of human cancers demonstrate alterations in microRNA expression. We hypothesized that regulatory defects in microRNAs play a central early role in organizing the molecular changes involved in ovarian cancer (OvCa). Using both gene arrays and deep sequencing, we comprehensively profiled mRNA and microRNA expression, respectively, in human serous epithelial OvCa cell lines, serous tumors, and short-term primary cultures of normal ovarian surface epithelium (NOSE). We expected that over-expression of a specific microRNA would lead to lower expression of its mRNA targets, and under-expression of a specific microRNA would lead to higher expression of its target genes. Using our expression data in conjunction with established in silico algorithms, we found putative microRNA:mRNA functional pairs. Furthermore, gene expression profiles were taken of serous cultures having functional knockdown or over-expression of specific microRNAs of interest. Over-expression of mir-31 (found under-expressed in serous OvCa) resulted in down-regulation in vitro of a significant number of the in silico predicted mir-31 target genes. Keywords: two group comparison Overall design: We used miRNA mimics to over-express mir-31, originally found under-expressed in OvCa. After 48 hours of mimic treatment, we profiled for gene expression both cells with mir-31 knockup and cells with mimic control (having minimal sequence identity with all human miRNAs).

INSTRUMENT(S): Illumina HumanHT-12 V3.0 expression beadchip

SUBMITTER: Chad Creighton   

PROVIDER: GSE16700 | GEO | 2010-02-25

SECONDARY ACCESSION(S): PRJNA122799

REPOSITORIES: GEO

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Publications

Molecular profiling uncovers a p53-associated role for microRNA-31 in inhibiting the proliferation of serous ovarian carcinomas and other cancers.

Creighton Chad J CJ   Fountain Michael D MD   Yu Zhifeng Z   Nagaraja Ankur K AK   Zhu Huifeng H   Khan Mahjabeen M   Olokpa Emuejevoke E   Zariff Azam A   Gunaratne Preethi H PH   Matzuk Martin M MM   Anderson Matthew L ML  

Cancer research 20100223 5


MicroRNAs (miRNA) regulate complex patterns of gene expression, and the relevance of altered miRNA expression to ovarian cancer remains to be elucidated. By comprehensively profiling expression of miRNAs and mRNAs in serous ovarian tumors and cell lines and normal ovarian surface epithelium, we identified hundreds of potential miRNA-mRNA targeting associations underlying cancer. Functional overexpression of miR-31, the most underexpressed miRNA in serous ovarian cancer, repressed predicted miR-3  ...[more]

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