Translational Changes Upon Dietary Restriction in Drosophila
Ontology highlight
ABSTRACT:
ORGANISM(S): Drosophila melanogaster
PROVIDER: GSE16738 | GEO | 2009/10/02
SECONDARY ACCESSION(S): PRJNA117431
REPOSITORIES: GEO
Similar Datasets
Project description:Analysis of the translational changes induced upon DR in male Drosophila. Experiment Overall Design: 1-2 day old flies were transferred onto 4% or 0.25% YE food for 6 days. ~100 male flies were then ground and RNA was separated by size on a sucrose gradient and fractionated using a Teledyne density gradient fractionator and a polysome profile was produced by monitoring the abs at 252nm. RNA was then prepared from the low (1-4 ribosomes bound) and high (5 or more ribosomes bound) translation fractions. A translational index was then calculated for each mRNA. The changes in translational index upon DR were then evaluated. This was done in triplicate on individual biological replicates.
2009-10-09 | E-GEOD-16738 | biostudies-arrayexpress
Project description:Aging is a complex multifactorial phenomenon largely driven by damaged macromolecules. We showed recently that with aging time- and exposure-dependent accumulation of DNA damage derails the basal process of transcription physically stalling RNA polymerase, lowering and skewing the transcriptional landscape in a gene-length-dependent fashion. However, how this influences the translational output and whether translation is similarly affected is largely unknown. Here, we present a parallel analysis of transcriptional and translational liver profiles from the well-characterized Ercc1Δ/− progeroid, DNA repair-deficient mouse model compared to wildtype under ad libitum conditions and upon dietary restriction (DR), which strongly delays aging in this mutant. Using ribosome profiling, we found that transcriptional changes during accelerated, normal, and delayed aging are largely preserved at the translational level ruling out a major translational impact on gene expression in aging. Moreover, in both Ercc1Δ/− and aged wild-type mice there was a prioritization of inflammation, metabolic redesign, and expression of translation initiation factors, along with a shift in codon occupancy. While translation initiation factors were further increased by DR, codon occupancy was partially normalized showing a discordant response. Additionally, increased ribosomal pausing and a relative reduction of upstream open reading frame expression were both further intensified by DR. Together these data infer a finetuning of the translational output, e.g. by regulating upstream open reading frames under various cellular stress situations. This study uncovers a complex interplay between DR, DNA damage, aging, and translational regulation, highlighting the potential of DR to modify DNA damage-driven translational dynamics during aging.
2026-04-20 | GSE288427 | GEO
Project description:Aging is a complex multifactorial phenomenon largely driven by damaged macromolecules. We showed recently that with aging time- and exposure-dependent accumulation of DNA damage derails the basal process of transcription physically stalling RNA polymerase, lowering and skewing the transcriptional landscape in a gene-length-dependent fashion. However, how this influences the translational output and whether translation is similarly affected is largely unknown. Here, we present a parallel analysis of transcriptional and translational liver profiles from the well-characterized Ercc1Δ/− progeroid, DNA repair-deficient mouse model compared to wildtype under ad libitum conditions and upon dietary restriction (DR), which strongly delays aging in this mutant. Using ribosome profiling, we found that transcriptional changes during accelerated, normal, and delayed aging are largely preserved at the translational level ruling out a major translational impact on gene expression in aging in our models. Moreover, in both Ercc1Δ/− and aged wild-type mice there was a prioritization of inflammation, metabolic redesign, and expression of translation initiation factors, along with a shift in codon occupancy. While translation initiation factors were further increased by DR, codon occupancy was partially normalized showing a discordant response. Additionally, increased ribosomal pausing and a relative reduction of upstream open reading frame expression were both further intensified by DR. Together these data infer a finetuning of the translational output, e.g. by regulating upstream open reading frames under various cellular stress situations. This study uncovers a complex interplay between DR, DNA damage, aging, and translational regulation, highlighting the potential of DR to modify DNA damage-driven translational dynamics during aging.
2026-04-20 | GSE288421 | GEO
Project description:The beneficial effects of dietary restriction (DR) are associated with a rearrangement of gene expression that modulate metabolic and cytoprotective pathways. However, the effect of DR on the cerebellar transcriptome remained to be fully defined. Therefore we analyzed the effect of 30% DR on the transcriptome of cerebellar cortex of young-adult male mice using RNAseq.
2023-06-14 | GSE228418 | GEO
Project description:Circadian clocks may mediate lifespan extension by caloric or dietary restriction (DR). We find that the core clock transcription factor Clock is crucial for a robust longevity and fecundity response to DR in Drosophila. To identify clock-controlled mediators, we performed RNA-sequencing from abdominal fat bodies across the 24 h day after just 5 days under control or DR diets. In contrast to more chronic DR regimens, we did not detect significant changes in the rhythmic expression of core clock genes. Yet we discovered that DR induced de novo rhythmicity or increased expression of rhythmic clock output genes. Network analysis revealed that DR increased network connectivity in one module comprised of genes encoding proteasome subunits. Adult, fat body specific RNAi knockdown demonstrated that proteasome subunits contribute to DR-mediated lifespan extension. Thus, clock control of output links DR-mediated changes in rhythmic transcription to lifespan extension. Funding: DARPA D12AP00023
2026-03-10 | GSE145509 | GEO
Project description:Living organisms adjust their proteome in response to nutritional conditions, but how these changes are transmitted across generations remains unclear. While unicellular organisms can inherit proteome states through symmetric division, multicellular organisms face limitations due to the soma-germline barrier. Using TMT-based quantitative proteomics, we investigated the intergenerational effects of dietary restriction (DR) on proteome allocation in C. elegans. While DR caused substantial proteome-wide changes within a generation, most of these changes were reset in the next generation. However, ribosomal proteins remained significantly reduced in progeny of DR mothers. This ribosomal reduction leads to an initial growth delay when encountering nutrient-rich conditions. This dataset provides a comprehensive resource to explore how proteome changes induced by DR are inherited across generations and to identify regulatory factors involved in ribosome inheritance and growth adaptation.
2026-02-10 | PXD060999 | Pride
Project description:Dietary restriction (DR) is a robust environmental intervention that slows aging in various species. Changes in fat content have been associated with DR, but whether they play a causal role in mediating various responses to DR remains unknown. We demonstrate that upon DR, Drosophila melanogaster shift their metabolism towards increasing both fatty acid synthesis and breakdown. Inhibition of acetyl CoA carboxylase (ACC), a critical enzyme in fatty acid synthesis, or fatty acid oxidation genes specifically in the muscle tissue inhibited the lifespan extension observed upon DR, suggesting a critical role for intra-myocellular fatty acid metabolism. DR enhances spontaneous activity of flies which was found to be dependent on the enhanced fatty acid metabolism. Furthermore, this increase in activity upon DR was found to partially mediate the lifespan extension upon DR. Over-expression of adipokinetic hormone (dAKH) in whole flies, which increases fat metabolism, led to an increase in spontaneous activity and lifespan in a nutrient dependent manner. Together these results suggest that in Drosophila melanogaster enhanced fat metabolism in the muscle is a key metabolic adaptation in response to DR.
2012-06-15 | GSE37537 | GEO
Project description:Dietary restriction (DR) is one of the most studied interventions known to extend life span. The robustness of its effect across species suggests the existence of conserved mechanisms to reduce mortality rates and increase longevity. However, because DR elicits a large number of physiological changes, many of which are unrelated to the longevity response, it has been difficult to identify these specific mechanisms. Whole-genome gene expression studies have typically reported several hundreds to thousands of differentially expressed genes in response to DR. The fruit fly Drosophila melanogaster shows a remarkable response to a change in diet: after a switch to DR, food mortality rates drop within 2-4 days to the same level as cohorts continuously on DR. Based on this observation, we utilized a novel experimental design to enrich for genes directly associated with the longevity response. By profiling gene expression in a cohort of fruit flies that were switched from normal food to DR we were able to partition genes in several classes with distinct patterns of expression.
2014-06-01 | GSE47631 | GEO
Project description: Not available
| S-EPMC2745347 | biostudies-literature
Project description:Translational changes upon aging and dietary restriction in progeroid DNA-repair-deficient mice
| PRJNA1217452 | ENA