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Polybrominated diphenyl ethers (PBDEs) exposure during pregnancy alters microRNA expression profile in rat placenta

ABSTRACT: Polybrominated diphenyl ethers (PBDEs) are well-known endocrine disrupting chemicals (EDCs), and have been found to affect reproduction and development. The placenta, as the interface between maternal and fetal circulation, is constantly exposed to maternal EDCs. The present study investigated PBDEs-induced changes in miRNAs in rat placenta, and the potential mechanism of developmental toxicity. Female rats were exposed to 500 mg/kg/day decabromodiphenyl ether (BDE-209) from gestational day 0 (GD 0) to GD 20. miRNAs were isolated from the placenta on GD 20. Expression of miRNAs were analyzed using next-generation sequencing, and validated by reverse transcription-quantitative polymerase chain reaction (RT-qPCR). A total of 55 miRNAs were found changed significantly in the exposure group, among which 46 were down-regulated and 9 were up-regulated. Rno-miR-17-5p, rno-miR-503-5p, rno-miR-374-5p, rno-miR-369-3p, rno-miR-29a-3p were confirmed to be down-regulated, rno-miR-148a-3p was validated up-regulated in the exposure group. The bioinformatic analysis showed that the predicted miRNAs target genes were involved in vascular endothelial growth factor (VEGF) signaling pathway, suggesting the role of PBDEs-induced developmental toxicity via the interference with the angiogenesis process in placenta. Our findings demonstrated evidence of altered microRNA expression with PBDEs exposure, thus providing novel insights into mechanisms of PBDEs developmental toxicity.

ORGANISM(S): Rattus norvegicus

PROVIDER: GSE169613 | GEO | 2025/12/31

REPOSITORIES: GEO

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Project description:We tested the hypothesis that a panel of placental mammal-specific miRNAs and their targets play important to establish receptivity to implantation and their dysregulated expression may be a feature in women with early pregnancy loss. Relative expression levels of miR-340-5p, −542-3p, and −671-5p all increased following treatment of Ishikawa cells with progesterone (10 μg/ml) for 24 hrs (p < 0.05). RNA sequencing of these P4-treated cells identified co-ordinate changes to 6,367 transcripts of which 1713 were predicted targets of miR-340-5p, 670 of miR-542-3p, and 618 of miR-671-5p. Quantitative proteomic analysis of Ishikawa cells transfected with mimic or inhibitor (48 hrs: n=3 biological replicates) for each of the P4-regulated miRNAs was carried out to identify targets of these miRNAs. Excluding off target effects, mir-340-5p mimic altered 1,369 proteins while inhibition changed expression of 376 proteins (p < 0.05) of which, 72 were common to both treatments. A total of 280 proteins were identified between predicted (mirDB) and confirmed (in vitro) targets. In total, 171 proteins predicted to be targets by mirDB were altered in vitro by treatment with miR-340-5p mimic or inhibitor and were also altered by treatment of endometrial epithelial cells with P4. In vitro targets of miR-542-3p identified 1,378 proteins altered by mimic while inhibition altered 975 a core of 200 proteins were changed by both. 100 protein targets were predicted and only 46 proteins were P4 regulated. miR-671-mimic altered 1,252 proteins with inhibition changing 492 proteins of which 97 were common to both, 95 were miDB predicted targets and 46 were also P4-regulated. All miRNAs were detected in endometrial biopsies taken from patients during the luteal phase of their cycle, irrespective of prior or future pregnancy outcomes Expression of mir-340-5p showed an overall increase in patients who had previously suffered a miscarriage and had a subsequent miscarriage, as compared to those who had infertility or previous miscarriage and subsequently went on to have a life birth outcome. The regulation of these miRNAs and their protein targets regulate the function of transport and secretion, and adhesion of the endometrial epithelia required for successful implantation in humans. Dysfunction of these miRNAs (and therefore the targets they regulate) may contribute to endometrial-derived recurrent pregnancy loss in women.

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Polybrominated diphenyl ethers (PBDEs) exposure during pregnancy alters microRNA expression profile in rat placenta

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