Transcriptomics,Genomics

Dataset Information

34

12h pathway stimulation / inhibition experiment in mouse ES cells


ABSTRACT: This experiment records the transcriptional responses of mES cells (line OG2) to FGF/ERK stimulation in the presence of LIF, to LIF/STAT3 inhibition in the presence of an FGF/ERK inhibitor, and to combined FGF/ERK stimulation / LIF/STAT3 inhibition. Overall design: ES cells were grown feeder-free on gelatin in KSR medium containing an FGF/ERK inhibitor and LIF and were then treated for 12h as indicated for the individual samples.

INSTRUMENT(S): Illumina MouseRef-8 v2.0 expression beadchip

SUBMITTER: Boris Greber  

PROVIDER: GSE17136 | GEO | 2010-02-21

SECONDARY ACCESSION(S): PRJNA123655

REPOSITORIES: GEO

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Publications

Conserved and divergent roles of FGF signaling in mouse epiblast stem cells and human embryonic stem cells.

Greber Boris B   Wu Guangming G   Bernemann Christof C   Joo Jin Young JY   Han Dong Wook DW   Ko Kinarm K   Tapia Natalia N   Sabour Davood D   Sterneckert Jared J   Tesar Paul P   Schöler Hans R HR  

Cell stem cell 20100301 3


Mouse epiblast stem cells (EpiSCs) are cultured with FGF2 and Activin A, like human embryonic stem cells (hESCs), but the action of the associated pathways in EpiSCs has not been well characterized. Here, we show that activation of the Activin pathway promotes self-renewal of EpiSCs via direct activation of Nanog, whereas inhibition of this pathway induces neuroectodermal differentiation, like in hESCs. In contrast, the different roles of FGF signaling appear to be only partially conserved in th  ...[more]

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