Transcriptomics,Genomics

Dataset Information

24

12h pathway stimulation / inhibition experiment in epiblast stem cells


ABSTRACT: This experiment records the transcriptional responses of EpiSCs (line E3) to FGF/ERK inhibition, GSK3ß inhibition, LIF/STAT3 activation, as well as combined treatment for 12 hours each. Overall design: EpiSCs were grown in KSR medium on low-density feeders. At semiconfluency, media were changed as detailed for the individual samples. Cells were harvested for RNA isolation 12 hours later.

INSTRUMENT(S): Illumina MouseRef-8 v2.0 expression beadchip

SUBMITTER: Boris Greber  

PROVIDER: GSE17134 | GEO | 2010-02-21

SECONDARY ACCESSION(S): PRJNA123653

REPOSITORIES: GEO

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Publications

Conserved and divergent roles of FGF signaling in mouse epiblast stem cells and human embryonic stem cells.

Greber Boris B   Wu Guangming G   Bernemann Christof C   Joo Jin Young JY   Han Dong Wook DW   Ko Kinarm K   Tapia Natalia N   Sabour Davood D   Sterneckert Jared J   Tesar Paul P   Schöler Hans R HR  

Cell stem cell 20100301 3


Mouse epiblast stem cells (EpiSCs) are cultured with FGF2 and Activin A, like human embryonic stem cells (hESCs), but the action of the associated pathways in EpiSCs has not been well characterized. Here, we show that activation of the Activin pathway promotes self-renewal of EpiSCs via direct activation of Nanog, whereas inhibition of this pathway induces neuroectodermal differentiation, like in hESCs. In contrast, the different roles of FGF signaling appear to be only partially conserved in th  ...[more]

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