Project description:Transcriptional profiling of 1μg/mL dox and 5μM 1NMPP1 treatedT-REx 293 cells expressing either WT or I642G IRE1α vs control. Overall design: TREx 293_WT_8h_03 and TREx 293_WT_NT_03 are hybed against a universal control sample;TREx 293_WT_24h_01and TREx 293_WT_NT_01 are hybed against a universal control smaple;TREx 293_WT_24h_02 and TREx 293_WT_NT_02 are hybed against a universal control smaple;TREx 293_IG_24h_01 and TREx 293_IG_NT_01 are hybed against a universal control sample;TREx 293_IG_24h_02 and TREx 293_IG_NT_02 are hybed against a universal control sample. The rest arrays are hybed pairwise with treated vs untreated on the same array.
Project description:Transcriptional profiling of INS1 cells expressing either WT IRE1α, I642G IRE1α, N906A IRE1α, or spliced XBP1 comparing 1μg/mL dox and 5μM 1NMPP1 treated vs control. All arrays are hybed pairwise with treated hybed against untreated
Project description:Transcriptional profiling of INS1 cells expressing either WT IRE1α, I642G IRE1α, N906A IRE1α, or spliced XBP1 comparing 1μg/mL dox and 5μM 1NMPP1 treated vs control. Overall design: All arrays are hybed pairwise with treated hybed against untreated
Project description:Transcriptional profiling of WT MEFs or IRE1α-/- MEFs treated with 1μM thapsigargin for 12hrs compared with their corresponding non-treated cells. Overall design: All arrays are hybed pairwise with treated against untreated
Project description:PGF (Placental growth factor) is a member of the vascular endothelial growth factor (VEGF) sub-family a crucial factor in angiogenesis and vasculogenesis. Mechanisms by which PlGF expression is regulated remain to be investigated in diabetic retinopathy DR. However, the underlying molecular mechanisms that PlGF, mediates in the early complications at non-proliferative DR remain mostly elusive. Here we have applied an LC-MS/MS-based label-free quantification proteomic approach to PlGF ablation in the retinal tissues from mouse strains (Akita, PlGF-/- and Akita.PlGF-/-). The proteomes of retinal tissues were extracted, digested by the Trypsin/LysC enzyme and subsequently analyzed by a Q-Exactive hybrid Quadrupole-Orbitrap mass spectrometer. We have performed normalization by the Z-score method, correlation by Pearson correlation coefficient and identified differentially expressed proteins in four comparisons. The gene ontology, functional pathways, and protein-protein network interaction analysis suggested that Gnb1, Gnb2, Gnb4, Gnai2, Gnao1, Snap2 and Gngt1 proteins are involved in insulin resistance pathways, which are down-regulated/ no significant in PlGF ablation in Akita diabetics (Akita.PlGF-/- vs. Akita), up-regulated in Akita vs. C57, PlGF-/- vs. C57. Prdx6, Map2 are involved in antioxidant activity and neural protection pathways respectively, which are up-regulated in Akita.PlGF-/- vs. Akita. Our proteomics outcomes anticipated that down-regulated of insulin resistance pathways, up-regulation of antioxidant and neuroprotection proteins might epitomize the potential mechanisms of anti-PlGF therapies in the treatment of DR.
Project description:Pairwise comparison of gene expression in pools (n=14) of wildtype 4 week old male Vs NaS1-/- knockout mice. Keywords: normal Vs mutant Overall design: Pairwise comparison: 4 technical replicates, 2 dye swaps