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Single-cell RNA-seq of UVB-radiated Skin Reveals Landscape of Photoaging-related Inflammation and Protection by Vitamin D

ABSTRACT: Due to the destruction of the ozone layer, the ultraviolet radiation received by our skin has increased significantly. Continuous exposure to sunlight can cause damage to our skin, which can lead to photoaging and even skin cancer. We performed single-cell RNA sequencing on the skin of normal and UV-irradiated mice, and found that UV-irradiation caused an inflammatory response in the skin of mice. On the contrary, the inflammation of the mice injected with vitamin D was significantly reduced. Finally, we uncovered that Hmox1 and vitamin D is related to achieve the anti-inflammatory effect of vitamin D. This provides a strategy for skin anti-UV treatment

ORGANISM(S): Mus musculus

PROVIDER: GSE173385 | GEO | 2022/05/16

REPOSITORIES: GEO

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Transcriptomics Expression Analysis of the effects of UV radiations on Normal Human Dermal Fibroblast Cells

Project description:Chronic exposure to sunlight is linked to premature aging (photoaging) and UV radiations is implicated to be the main contributor of this phenomenon. It causes changes to dermal collgen through two major pathways; promotion of collagen breakdown and lost of collagen content via the inhibition of collagen biosynthesis. The absorption of UV radiations by skin molecules generates reactive oxygen species (ROS) which lead to oxidative damage of cellular components such as cell walls, lipid membranes, mitochondria and DNA. Therefore, this research involve the identification of key genes in biological processes through high throughput RNA Sequencing technology to identify genes and pathways underlying skin aging health and diseases due to UV exposure, ultimately leading to novel ways of managing, diagnose, treat and prevent/slow down skin aging illnesses/wrinkles.The results from this study produces huge amount of differential expressed genes (DEG) as a result of UV exposure which were subsquently validated with qPCR.

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2024-01-16 | GSE251675 | GEO

Single-cell RNA-seq of UVB-radiated Skin Reveals Landscape of Photoaging-related Inflammation and Protection by Vitamin D

Project description:Single-cell RNA-seq of UVB-radiated Skin Reveals Landscape of Photoaging-related Inflammation and Protection by Vitamin D

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2022-02-17 | PXD025973 | Pride

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Cxcl1 antagonist on UVB-induced skin inflammation leading to inhibitory effects on photo-skin tumorigensis in Xeroderma pigmentosum type A deficient mice

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2017-06-01 | GSE86093 | GEO

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A single dose of Ultraviolet-A induces proteome remodeling and senescence in primary human keratinocytes

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2022-02-17 | PXD025191 | Pride

Alterations in Extracellular Matrix Composition during Aging and Photoaging of the Skin

Project description:Human skin is composed of the cell-rich epidermis, the extracellular matrix (ECM) rich dermis, and the hypodermis. Within the dermis, a dense network of ECM proteins provides structural support to the skin and regulates a wide variety of signaling pathways which govern cell proliferation and other critical processes. Both intrinsic aging, which occurs steadily over time, and extrinsic aging (photoaging), which occurs as a result of external insults such as UV radiation, cause alterations to the dermal ECM. In this study, we utilized both quantitative and global proteomics, alongside single harmonic generation (SHG) and two-photon autofluorescence (TPAF) imaging, to assess changes in dermal composition during intrinsic and extrinsic aging. We find that both intrinsic and extrinsic aging result in significant decreases in structural ECM integrity, evidenced by decreasing collagen abundance and increasing fibril fragmentation, and ECM-supporting proteoglycans. Intrinsic aging also produces changes distinct from those produced by photoaging, including reductions in elastic fiber and crosslinking enzyme abundance. In contrast, photoaging is primarily defined by increases in elastic fiber-associated protein and pro-inflammatory proteases. Changes induced by photoaging are evident even in comparisons of young underarm and forearm skin, indicating that photoexposure experienced by an individual’s mid-20s is sufficient for large-scale proteomic alterations and that molecular-level changes due to photoaging are evident well before clinical indications are present. GO term enrichment revealed that both intrinsic aging and photoaging share common features of chronic inflammation.

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