Project description:To identify candidate downstream mRNA target for hsa-miR-130b miR-130b or empty vector control was overexpressed in PLC8024 CD133- HCC cells using lentivirus
Project description:We report transcriptomic changes in human umbilical vein endothelial cells after transfection of negative control mimic and hsa-miR-130b-3p mimic.
Project description:To identify candidate downstream mRNA target(s) for hsa-miR-616 Affymetrix Human Genome U133 Plus GeneChip 2.0 miR-616 or empty vector control was stably overexpressed in LNCaP prostate cells using lentivirus
Project description:Hsa-mir-365-2 is one of the two precursors that give rise to miR-365. We discovered that miR-365 directly regulates a lung cancer and developmental gene termed thyroid transcription factor 1 (TTF-1 or NKX2-1). Hsa-mir-365-2 was transfected into NCI-H441 cells via retrovirus-mediated gene transfer. Arrays hybridized: Affymetrix GeneChip Human Gene 1.0 ST Array.
Project description:Hsa-mir-365-2 is one of the two precursors that give rise to miR-365. We discovered that miR-365 directly regulates a lung cancer and developmental gene termed thyroid transcription factor 1 (TTF-1 or NKX2-1). Hsa-mir-365-2 was transfected into NCI-H441 cells via retrovirus-mediated gene transfer. Arrays hybridized: Affymetrix GeneChip Human Gene 1.0 ST Array. A total of 4 samples were analyzed. The first two were H441 cells expressing hsa-mir-365-2 and the other two were H441 transfected with empty vector. The class comparison function (significant cutoff, 0.001) of BRB Array Tool (4.1.0 beta_3 release) was used to analyze for differentially expressed genes as a result of enforced expression of hsa-mir-365-2.
Project description:Estrus is a critical phase in the reproductive cycle of sows and depends on normal ovarian development and steroid hormone secretion. Granulosa cells (GCs) play crucial roles in maintaining normal ovarian physiological functions in sows by secreting estradiol (E2) and progesterone (P4). CircRNAs influence steroid hormone synthesis, but their involvement in regulating gilt estrous remains unclear. In this study, circRNA sequencing was performed on the ovaries of estrus (ES) and nonestrus (NES) gilts, leading to the identification of a novel circRNA, termed circSHOC2, which is highly expressed in ES ovaries. The overexpression of circSHOC2 promoted the synthesis of E2 and P4 and increased the protein levels of key steroidogenic enzymes. Further investigation revealed that circSHOC2 acts as a sponge for miR-130b-5p. Silencing miR-130b-5p significantly enhanced E2 and P4 production, along with the upregulation of steroidogenic proteins. Additionally, miR-130b-5p was found to target ASH1 like histone lysine methyltransferase (ASH1L), with miR-130b-5p overexpression significantly inhibiting ASH1L expression. Cotransfection experiments revealed that ASH1L mitigated the inhibitory effects of miR-130b-5p on E2 and P4 synthesis in GCs. This study, through circRNA sequencing of ovaries from ES and NES gilts, revealed that circSHOC2 regulates steroid hormone synthesis in porcine GCs via the miR-130b-5p/ASH1L axis, shedding light on a potential molecular pathway involved in gilts estrus. Our results will provide molecular targets for the selection of excellent estrus sows, thereby improving the utilization rate of gilts in estrus.
Project description:Analysis of miR-130b regulated genes in TC71 Ewing Sarcoma cells. The hypothesis tested being that overexpression of miR-130b increases metastasis in Ewing Sarcoma.