Detection of aberrant DNA methylation in AML cell lines compared to normal human monocytes

ABSTRACT: To globally define methylation-’prone’ and -’protected’ CpG islands in leukemia, we analyzed the methylation status of 23,000 CpG islands of the human genome in two acute leukemia cell lines as well as normal blood monocytes using our previously described methyl-CpG immunoprecipitation (MCIp) technique (Gebhard et al. 2006; Schilling and Rehli 2007). This method enriches for highly CpG methylated DNA that can be directly applied to fluorescent labeling and oligonucleotide microarray hybridization without an additional amplification step. Keywords: MCIp-on-Chip; comparative genomic hybridization

ORGANISM(S): Homo sapiens

PROVIDER: GSE17455 | GEO | 2010/03/31

SECONDARY ACCESSION(S): PRJNA118727

REPOSITORIES: GEO