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Single-cell analysis identifies a key role for Hhip in murine coronal suture development

ABSTRACT: Craniofacial development depends on formation and maintenance of sutures between bones of the skull. In sutures, growth occurs at osteogenic fronts along the edge of each bone and suture mesenchyme separates adjacent bones. We performed single-cell RNA-seq analysis of the embryonic, murine coronal suture. Seven populations at E16.5 and nine at E18.5 comprised the suture mesenchyme, osteogenic cells, and associated populations. Expression of Hhip, an inhibitor of hedgehog (HH) signaling, marked a mesenchymal population distinct from other neurocranial sutures. At E18.5, Hhip-/- coronal osteogenic fronts were closely apposed and HH signaling was increased throughout the depleted suture mesenchyme compared to WT, demonstrating that Hhip is required for normal coronal suture development. Tracing of the neonatal Hhip-expressing population showed that descendant cells persisted in the coronal suture and contributed to calvarial bone growth. Our transcriptomic approach provides a rich resource for insight into normal and abnormal development.

ORGANISM(S): Mus musculus

PROVIDER: GSE178899 | GEO | 2021/06/26

REPOSITORIES: GEO

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The developing mouse coronal suture at single-cell resolution

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Skeletal Stem Cell Powering Cranial Suture Fate: An Answer to Craniosynostosis (scRNA-seq)

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Single-cell analysis identifies a key role for Hhip in murine coronal suture development

Project description:Single-cell analysis identifies a key role for Hhip in murine coronal suture development

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