Next Generation Sequencing (m6A-seq) Reveals RNA m6A Methylation Patterns During the Expansion of Tomato

ABSTRACT: In this study, we show the potential link between m6A deposition and fruit expansion in tomato (Micro-tom). m6A level was increased during fruit expansion. Later, a parallel analysis of the m6A -seq and RNA-seq in expanding fruit were preformed to evaluate global correlation between m6A modification and fruit expansion. Our m6A -seq reveal that thousands of protein-coding genes contains m6A modification with a consensus motif and primarily enriched in the 3’untranslated regions. Gene ontology analysis indicate that the distribution of m6A in expanded fruit is largely associated with plant-specific processes relating to the chloroplast function. Moreover, we further uncover a positive correlation between m6A methylation and the mRNA abundance. Specifically, large number of fruit expansion-related genes, which involved in hormone and endoreplication, were m6A modified and actively expressed compared to non- m6A modified genes, suggesting a potential involvement of m6A modification in fruit expansion. Collectively, our result suggests a dynamic role of m6A modification involving in fruit expansion.

ORGANISM(S): Solanum lycopersicum

PROVIDER: GSE178904 | GEO | 2021/07/03

REPOSITORIES: GEO