Transcriptomics,Genomics

Dataset Information

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MicroRNA-sequencing of plasma from patients in the Osteoarthritis Initiative Cohort


ABSTRACT: Objective: we aimed to identify circulating microRNAs associated with fast-progressing knee osteoarthritis (OA) as compared to slow-progressing knee OA and non-progressing knee OA using sujects from the Osteoarthritis Initiative (OAI) cohort. MicroRNA libraries were prepared from plasma using the QIAseq miRNA Library Kit (QIAGEN) and sequenced on the Illumina NextSeq550 using a single-end 75-base read protocol to an average depth of 11.6 ± 2.6 SD million reads per sample. Overall design: MicroRNA-sequencing was performed on plasma collected at baseline and 4-year follow-up from N=20 fast-progressors (Kellgren-Lawrence [KL] grade 0/1 at baseline to KL 3/4 by 4-year follow-up), N=35 slow-progressors (KL 0/1 at baseline and 4-year follow-up to KL 2/3/4 by 8-year follow-up), and N=51 non-progressors (KL 0/1 at baseline through to 8-year follow-up).

INSTRUMENT(S): NextSeq 550 (Homo sapiens)

SUBMITTER: Mohit Kapoor 

PROVIDER: GSE183188 | GEO | 2022-03-31

REPOSITORIES: GEO

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Publications

Circulating microRNAs differentiate fast-progressing from slow-progressing and non-progressing knee osteoarthritis in the Osteoarthritis Initiative cohort.

Ali Shabana Amanda SA   Espin-Garcia Osvaldo O   Wong Andy K AK   Potla Pratibha P   Pastrello Chiara C   McIntyre Madison M   Lively Starlee S   Jurisica Igor I   Gandhi Rajiv R   Kapoor Mohit M  

Therapeutic advances in musculoskeletal disease 20220318


<h4>Introduction</h4>The objective of this study is to identify circulating microRNAs that distinguish fast-progressing radiographic knee osteoarthritis (OA) in the Osteoarthritis Initiative cohort by applying microRNA-sequencing.<h4>Methods</h4>Participants with Kellgren-Lawrence (KL) grade 0/1 at baseline were included (<i>N</i> = 106). Fast-progressors were defined by an increase to KL 3/4 by 4-year follow-up (<i>N</i> = 20), whereas slow-progressors showed an increase to KL 2/3/4 only at 8-y  ...[more]

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