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Functional genomic analysis of the SPL9 gene in Arabidopsis thaliana under low phosphate conditions

ABSTRACT: Inorganic phosphate (Pi) is an essential nutrient, which is often served as a limiting factor in plant growth. It has been reported that SPL family members, such as SPL3, regulate Pi deficiency responses by controlling the expression of Pi deficiency responsive genes. To elucidate whether SPL9 respond to low phosphorus stress, we investigated the phenotypes and conduct RNA sequencing analysis in transgenic Arabidopsis thaliana with overexpressing SPL9 (R9) under conditions of both normal and low Pi availability. Compared with wild-type plants, R9 showed decreased anthocyanin accumulation and increased Pi contents in shoots under Pi deficiency. Through RNA-seq analysis compared with wild-type plants, we detected 217 genes significantly differentially expressed in conditions of Pi sufficiency, and 121 genes differentially expressed in conditions of Pi deficiency in R9 plants. Under Pi deficiency, these genes included multiple protein kinases, jasmonic acid response genes and genes related to salt stress responses. Genes associated with hydrolase and transferase activity were also differentially regulated by Pi deficiency, such as cytochrome P450 monooxygenases. Of particular note, the transcription factor AP2-EREBP and members of the bHLH family were among the most significantly differentially regulated genes identified under both Pi sufficient and Pi deficient conditions.

ORGANISM(S): Arabidopsis thaliana

PROVIDER: GSE183312 | GEO | 2021/09/03

REPOSITORIES: GEO

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Transcription profiling of Arabidopsis roots to investigate early phosphate-deficiency induced signal changes

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2009-04-18 | E-GEOD-15649 | biostudies-arrayexpress

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2012-11-29 | E-GEOD-31132 | biostudies-arrayexpress

Arabidopsis thaliana Raw sequence reads

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2015-06-20 | E-MTAB-4396 | biostudies-arrayexpress

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