Dataset Information


MicroRNA-target RNA pairs revealed by Parallel analysis of RNA ends in Vitis vinifera

ABSTRACT: Vitis vinifera RNA degradome Overall design: Isolated polyadenylated RNA from total RNA extracts of Vitis vinifera leaves, were ligated to 5'-adapter that include san MmeI recognition site. The ligated products were purified again, reverse transcribed and cleaved with MmeI. The 5' fragments were purified from gel and to a 3'- dsDNA adapter and PCR amplified. After PCR amplification the sample was subjected to Solexa/Illumina high throughput pyrosequencing. Please see for details of the sequencing technology.

INSTRUMENT(S): Illumina Genome Analyzer (Vitis vinifera)

ORGANISM(S): Vitis vinifera  

SUBMITTER: Simon Moxon   

PROVIDER: GSE18406 | GEO | 2010-04-29



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