Transcriptomics

Dataset Information

29

Gene Expression Changes in Campylobacter jejuni triggered by exposure to Novobiocin


ABSTRACT: In order to investigate the gene experssion changes in Campylobacter jejuni triggered by exposure to Novobiocin. Overall design: DNA microarray was used to identify genes that were differentially expressed in C. jejuni 11168 treated with Novobiocin. For RNA extraction, Campylobacter cells were grown 4 hrs in MH broth to the mid exponential phase (OD600=0.4-0.5) with shaking rates 180rpm at 42°C, and then split into two equal portions, one of which was treated with subinhibitory dose of Novobiocin (256ug/ml, 0.25 MIC) and the other served as a non-treated control, all of these samples were incubated at 42°C for 30 min under microaerobic conditions, RNA samples were extracted from 4 independent treatments and 4 non-treated controls. Then cDNA was synthesized using aminoallyl-dNTP and RNA samples according to the manufacturer’s instruction. After purification, aminoallyl labeled cDNA was fluorescently labeled with Cy-3/Cy-5 mono-Reactive Dye Pack.

INSTRUMENT(S): JCVI PFGRC Campylobacter jejuni 23K array [full array version]

ORGANISM(S): Campylobacter jejuni  

SUBMITTER: Yang Wang  

PROVIDER: GSE18415 | GEO | 2009-12-28

SECONDARY ACCESSION(S): PRJNA118207

REPOSITORIES: GEO

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