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Identification of molecular factors explaining decreased hypocotyl growth at low temperature

ABSTRACT: affy_hypoctemp_medicago - The characterization of several genotypes of the model Legume Medicago truncatula showed a genetic variability for germination and hypocotyl heterotrophic growth at low temperature and optimal temperature. The most important contrast was between the accessions Jemalong A17 and F83005.5. In order to find genes differently expressed between temperatures and genotypes, the present work focuses on transcriptome profiling during hypocotyl heterotrophic growth under low (10°C) and optimal (20°C) temperature conditions for both genotypes. We used Jemalong A17 and F83005.5 seeds coming from the Medicago truncatula Biological Resource Center in Montpellier, produced in 2006. Experiments were performed in the dark to mimic pre-emergence growth. Pots (6.5 cm diameter, 10 cm high) were incubated in growth chambers either at 10°C or 20°C. They were filled with 500g sand and 100ml of a nutrient solution for young seedlings growth (Saglio and Pradet, 1980). Per pot, five scarified seeds were sown at 1.5 cm depth. Seedlings were harvested at three times: 35°Cd (degree day), 50°Cd and 100°Cd. At each timepoint, about 50 seedlings were harvested and hypocotyl lengths was measured. Hypocotyl were cut and immediately frozen in liquid nitrogen for RNA extractions.

ORGANISM(S): Medicago sativa Sinorhizobium meliloti Medicago truncatula

PROVIDER: GSE18449 | GEO | 2010/09/10

SECONDARY ACCESSION(S): PRJNA118163

REPOSITORIES: GEO

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Identification of molecular factors explaining decreased hypocotyl growth at low temperature

Project description:affy_hypoctemp_medicago - The characterization of several genotypes of the model Legume Medicago truncatula showed a genetic variability for germination and hypocotyl heterotrophic growth at low temperature and optimal temperature. The most important contrast was between the accessions Jemalong A17 and F83005.5. In order to find genes differently expressed between temperatures and genotypes, the present work focuses on transcriptome profiling during hypocotyl heterotrophic growth under low (10°C) and optimal (20°C) temperature conditions for both genotypes. We used Jemalong A17 and F83005.5 seeds coming from the Medicago truncatula Biological Resource Center in Montpellier, produced in 2006. Experiments were performed in the dark to mimic pre-emergence growth. Pots (6.5 cm diameter, 10 cm high) were incubated in growth chambers either at 10°C or 20°C. They were filled with 500g sand and 100ml of a nutrient solution for young seedlings growth (Saglio and Pradet, 1980). Per pot, five scarified seeds were sown at 1.5 cm depth. Seedlings were harvested at three times: 35°Cd (degree day), 50°Cd and 100°Cd. At each timepoint, about 50 seedlings were harvested and hypocotyl lengths was measured. Hypocotyl were cut and immediately frozen in liquid nitrogen for RNA extractions. Time course of 24 arrays - Medicago

2010-09-10 | E-GEOD-18449 | biostudies-arrayexpress

Comparative proteomic analysis reveals differential root proteins in Medicago sativa and Medicago truncatula in response to salt stress

Project description:we used two-dimensional gel electrophoresis and mass spectrometry to characterize the proteome-level changes associated with salt stress response in Medicago sativa cv. Zhongmu-1 and Medicago truncatula cv. Jemalong A17 roots. The tandem mass spectrometry analysis of the differentially accumulated proteins resulted in the identification of 60 and 26 proteins in Zhongmu-1 and Jemalong A17 roots, respectively.

2016-04-14 | PXD003761 | Pride

Growth temperature response in Arabidopsis

Project description:Moderate increases in the ambient temperature promote hypocotyl growth in Arabidopsis, and this response is totally dependent on the proper activity of the auxin, gibberellin, and brassinosteroid pathways. We have analyzed global the changes in gene expression that occur in Arabidopsis seedlings after a moderate increase in the growth temperature (20ºC to 29ºC for 2 hours). In order to understand how the different hormone pathways affect this growth response, the same transcription profiling analysis was conducted in seedlings deficient in each hormone. Keywords: Growth condition

2009-07-27 | GSE13822 | GEO

Transcription profiling of root tips from Medicago truncatula Jemalong A17 and TN1.11 after salt stress

Project description:Effect of Salt Stress on root tip transcriptome of Medicago truncatula Jemalong A17 and Medicago truncatula TN1.11

2010-01-01 | E-MEXP-1255 | biostudies-arrayexpress

Transcription profiling of Medicago truncatula TTG1 over-expressing hairy root

Project description:Transcriptomics analysis of MtTTG1 over-expressing hairy root in the background of Medicago truncatula genotype Jemalong A17.

2009-08-14 | E-MEXP-1758 | biostudies-arrayexpress

Expression data from Medicago truncatula roots treated with S. meliloti wild-type or exoA mutant bacteria, or auxin transport inhibitors

Project description:Publication title: Pseudonodule formation by wild type and symbiotic mutant Medicago truncatula in response to auxin transport inhibitors This SuperSeries is composed of the following subset Series: GSE27991: Expression data of Medicago truncatula Jemalong A17 roots treated with auxin transport inhibitors GSE28171: Expression data of Medicago truncatula Jemalong A17 roots treated with S. meliloti exoA mutant or auxin transport inhibitors GSE28172: Expression data of Medicago truncatula skl1-1 roots treated with S. meliloti wild-type or auxin transport inhibitors GSE28173: Genes differentially expressed in wild-type Medicago truncatula plants during nodulation Refer to individual Series

2011-03-25 | E-GEOD-28174 | biostudies-arrayexpress

Transcriptomics of the plant growth regulator Heatin

Project description:Temperature passively affects many biological processes. It is therefore challenging to study dedicated temperature signalling pathways orchestrating plant thermomorphogenesis; a suite of elongation growth-based adaptations that enhance leaf cooling capacity. Following a chemical genetics approach, we screened a chemical library for compounds that restored abolished hypocotyl elongation in the pif4-2 deficient mutant background in the model plant Arabidopsis thaliana. The small aromatic compound ‘Heatin' (N'-[(2-hydroxy-1-naphthyl)methylene]-2-(1-naphthylamino)propanohydrazide), with 1-aminomethyl-2-naphthol, as minimal active moiety, was isolated as potent enhancer of elongation growth. Here we assessed the transcriptomic changes induced by the compound, by supplementing the MS-agar growth medium with Heatin compared to DMSO solvent, in whole seedlings of the Col-0 wild type background in control (22oC) and high ambient temperature conditions (27oC).

2019-12-01 | GSE130964 | GEO

Transcription profiling of Medicago truncatula after salt stress treatment to the root tips and untreated control plants

Project description:A Salt stress (100mM) was applied to root tips of Medicago truncatula (Jemalong A17 genotype) for 1h. Microarrays were used to compare the transcriptome of these plants (4 replicates) with the transcriptome of control plants, not submitted to the salt stress (4 replicates)

2009-01-14 | E-MEXP-1441 | biostudies-arrayexpress

B-box family proteins BBX18 and BBX23 control thermoresponsive hypocotyl growth in Arabidopsis

Project description:Plants coordinate their growth and developmental programs with various endogenous signals and environmental challenges such as seasonal and diurnal temperature fluctuations. The bHLH transcription factor PIF4 plays critical roles in thermoresponsive hypocotyl growth in Arabidopsis, and the evening complex component ELF3 negatively regulates PIF4's activity for downstream gene expression and hypocotyl elongation at elevated temperature. However, how warm temperature signal is transmitted to ELF3 is not known. Here, we report the identification of two B-Box protein BBX18/BBX23 as new regulators of thermomorphogenesis in Arabidopsis. Mutations of BBX18/BBX23 confer reduced thermoresponsive hypocotyl elongation. Overexpression of BBX18 enhances the sensitivity of hypocotyl growth to elevated temperature, which is dependent on the function of PIF4 and RING E3 ligase COP1, respectively. Both BBX18 and BBX23 interact with ELF3 or COP1, relegating the protein abundance of ELF3 at warm temperature. Further, the expression of multiple thermoresponsive genes is impaired in both the PIF4 single mutant and BBX18/BBX23 double mutant. In addition, both the transcription and protein levels of BBX18/BBX23 are up-regulated by elevated ambient temperature. Thus, our findings reveal the important roles of B-Box proteins in plant thermomorphogenesis, and build a new connection from warm temperature information to ELF3 and its downstream signaling components.

2018-12-21 | GSE108162 | GEO

Transcription profiling of Medicago truncatula with benzylaminopurine treatment to the root tips and untreated control plants

Project description:A BAP (benzylaminopurine, cytokinin) solution (10-7 M) was applied to root tips of Medicago truncatula (Jemalong A17 genotype) for 1h. Microarrays were used to compare the transcriptome of these plants (4 replicates) with the transcriptome of control plants, not exposed to BAP (4 replicates)

2010-01-01 | E-MEXP-1442 | biostudies-arrayexpress

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