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Comparison of DNA methylation profiles in diploid and transformed tetraploid MEF cells

ABSTRACT: We propose that tetraploidy induces epigentic changes including DNA methylation due to the abnormal chromatin in the cells. To test our hypothesis, we employed methylated CpG island recovery assay (MIRA) assisted microarray to determine the DNA methylation profiles in both diploid and tetraploid cells.

ORGANISM(S): Mus musculus

PROVIDER: GSE18814 | GEO | 2012/04/30

SECONDARY ACCESSION(S): PRJNA123857

REPOSITORIES: GEO

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Comparison of DNA methylation profiles in diploid and transformed tetraploid MEF cells

Project description:We propose that tetraploidy induces epigentic changes including DNA methylation due to the abnormal chromatin in the cells. To test our hypothesis, we employed methylated CpG island recovery assay (MIRA) assisted microarray to determine the DNA methylation profiles in both diploid and tetraploid cells. First, we conducted a methylated-CpG island recovery assay (MIRA). Briefly, we enriched for methylated CpG islands with methylated DNA binding proteins, MBD2/MBD3L1. The pulled down DNA fragments containing CpG islands and input DNA fragments were amplified with real-time PCR. After labeling, they were hybridized to CpG island promoter array. Data were collected and analyzed.

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Comparison of diploid and transformed tetraploid MEF cells: gene expression and DNA methylation profiling

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Project description:We propose that abnormal DNA content in tetraploid cells can alter the gene expression profile, contributing to the cellular transformation. To test this hypothesis, we employ mRNA microarray to compare the gene expression profile in transformed tetraploid cells with that in the parent diploid cells. Keywords: gene expression profile determination Total RNA were extracted from diploid or tetraploid cells (n = 3 for each cell lines. mRNA microarray hybridization was conducted. Data were collected and analyzed.

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