MicroRNA expression and opioid regulation in the zebrafish
Ontology highlight
ABSTRACT:
OTHER RELATED OMICS DATASETS IN: PRJNA121187
ORGANISM(S): Caenorhabditis elegans Rattus norvegicus Danio rerio Mus musculus Homo sapiens Drosophila melanogaster
PROVIDER: GSE18847 | GEO | 2010/12/01
SECONDARY ACCESSION(S): PRJNA121187
REPOSITORIES: GEO
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Project description:To study the relationship between microRNAs and μ-opioid receptor (MOR) signaling, we examined microRNA expression after chronic morphine or fentanyl treatment in rat primary hippocampal neurons and in mouse hippocampus. Mouse cerebellum region was also tested as a negative control to eliminate microRNA expression changes unrelated to MOR signaling, as the cerebellum is essentially devoid of MOR. We identified a number of microRNAs that altered their expression upon treatment with both morphine and fentanyl in the rat and mouse systems. There were, however, some microRNAs that changed in response to morphine, or fentanyl, but not both. Keywords: Expression profiling
2009-05-04 | GSE14268 | GEO
Project description:To study the relationship between microRNAs and μ-opioid receptor (MOR) signaling, we examined microRNA expression after chronic morphine or fentanyl treatment in rat primary hippocampal neurons and in mouse hippocampus. Mouse cerebellum region was also tested as a negative control to eliminate microRNA expression changes unrelated to MOR signaling, as the cerebellum is essentially devoid of MOR. We identified a number of microRNAs that altered their expression upon treatment with both morphine and fentanyl in the rat and mouse systems. There were, however, some microRNAs that changed in response to morphine, or fentanyl, but not both. Keywords: Expression profiling There are up to three biological replicates (indicated by 1, 2, and 3) of primary hippocampal neurons from new born rats and the cerebellum and hippocampus regions from adult mice treated for three days (control, morphine, and fentanyl). The biological replicates were from experiments performed on different dates. Each biological replicate contained cells or tissues collected from multiple animals so that enough RNA could be extracted for RNA analysis. RNA was labelled with a green dye, mixed with a reference DNA sample labelled with a red dye. The reference DNA contained a number of synthetic DNA oligos with mature microRNA sequences that served to verify microarray hybridization. RNA signals were in ch1, DNA signals ch2.
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Project description:microRNA expression and opioid regulation in the zebrafish
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Project description:A great number of studies have investigated changes induced by morphine exposure in gene expression using several experimental models. In this study, we examined gene expression changes during chronic exposure to morphine during maturation and differentiation of zebrafish CNS. Our study identified different functional classes of genes and individual candidates involved in the mechanisms underlying susceptibility to morphine actions related to CNS development. These results open new lines to study the treatment of pain and the molecular mechanisms involved in addiction. We also found a set of zebrafish-specific morphine-induced genes, which may be putative targets in human models for addiction and pain processes.
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Project description:Growing evidence shows that miRNAs play essential roles in cell development by regulating the growth and differentiation of cell lineages. However, the potential roles of miRNA-mediated regulation in the environmental adaptation of organisms are largely unknown. To examine this potential regulatory capability, we systematically identified and compared the miRNAs of zebrafish brains under normal and cold-acclimated conditions using Solexa sequencing. We then predicted the potential target protein genes of the miRNAs and compared their gene expression profile patterns derived from microarray analyses. We defined a set of 25 differentially expressed miRNAs between the cold-acclimatized and normal zebrafish and predicted their functions based on the GO annotation of their targets. We also compared the expression of mRNAs and found that genes related to mRNA processing and response to stress were overrepresented among the up-regulated genes of the cold-stress condition. We analyzed the potential regulatory roles of these miRNAs on gene transcription patterns under low-temperature conditions using several statistical models and a novel, network-based approach. Our results indicate that these miRNAs, either individually or together and either directly or indirectly mediated by transcription factors (TFs), contribute a minor amount to the change in gene expression patterns under low-temperature conditions. Adult zebrafish (Danio rerio) were raised under the normal laboratory breeding temperature (28C) or subjected subjected to cold (10C) stress. After 10 days of cold adaption, the brains of the zebrafish were dissected, and total RNAs were extracted from these brains. Expressional profiles of miRNAs and mRNAs were detected through high-throughput Solexa sequencing and microarray respectively. This submission represents transcriptome component of study
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Project description:Growing evidence shows that miRNAs play essential roles in cell development by regulating the growth and differentiation of cell lineages. However, the potential roles of miRNA-mediated regulation in the environmental adaptation of organisms are largely unknown. To examine this potential regulatory capability, we systematically identified and compared the miRNAs of zebrafish brains under normal and cold-acclimated conditions using Solexa sequencing. We then predicted the potential target protein genes of the miRNAs and compared their gene expression profile patterns derived from microarray analyses. We defined a set of 25 differentially expressed miRNAs between the cold-acclimatized and normal zebrafish and predicted their functions based on the GO annotation of their targets. We also compared the expression of mRNAs and found that genes related to mRNA processing and response to stress were overrepresented among the up-regulated genes of the cold-stress condition. We analyzed the potential regulatory roles of these miRNAs on gene transcription patterns under low-temperature conditions using several statistical models and a novel, network-based approach. Our results indicate that these miRNAs, either individually or together and either directly or indirectly mediated by transcription factors (TFs), contribute a minor amount to the change in gene expression patterns under low-temperature conditions.
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