MicroRNA expression and opioid regulation in the zebrafish
Ontology highlight
ABSTRACT:
OTHER RELATED OMICS DATASETS IN: GSE18847
PROVIDER: PRJNA121187 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:We examined morphine responsing miRNAs in Zebrafish embroys. Microarray studies revealed morphine can change microRNA expression in Zebrafish embroys.
2010-12-01 | GSE18847 | GEO
Project description:microRNA expression and opioid signaling
| PRJNA111231 | ENA
Project description:To study the relationship between microRNAs and μ-opioid receptor (MOR) signaling, we examined microRNA expression after chronic morphine or fentanyl treatment in rat primary hippocampal neurons and in mouse hippocampus. Mouse cerebellum region was also tested as a negative control to eliminate microRNA expression changes unrelated to MOR signaling, as the cerebellum is essentially devoid of MOR. We identified a number of microRNAs that altered their expression upon treatment with both morphine and fentanyl in the rat and mouse systems. There were, however, some microRNAs that changed in response to morphine, or fentanyl, but not both. Keywords: Expression profiling
2009-05-04 | GSE14268 | GEO
Project description:To study the relationship between microRNAs and μ-opioid receptor (MOR) signaling, we examined microRNA expression after chronic morphine or fentanyl treatment in rat primary hippocampal neurons and in mouse hippocampus. Mouse cerebellum region was also tested as a negative control to eliminate microRNA expression changes unrelated to MOR signaling, as the cerebellum is essentially devoid of MOR. We identified a number of microRNAs that altered their expression upon treatment with both morphine and fentanyl in the rat and mouse systems. There were, however, some microRNAs that changed in response to morphine, or fentanyl, but not both. Keywords: Expression profiling There are up to three biological replicates (indicated by 1, 2, and 3) of primary hippocampal neurons from new born rats and the cerebellum and hippocampus regions from adult mice treated for three days (control, morphine, and fentanyl). The biological replicates were from experiments performed on different dates. Each biological replicate contained cells or tissues collected from multiple animals so that enough RNA could be extracted for RNA analysis. RNA was labelled with a green dye, mixed with a reference DNA sample labelled with a red dye. The reference DNA contained a number of synthetic DNA oligos with mature microRNA sequences that served to verify microarray hybridization. RNA signals were in ch1, DNA signals ch2.
2010-05-19 | E-GEOD-14268 | biostudies-arrayexpress
Project description:The opioid epidemic has introduced significant public health challenges with little knowledge regarding the consequences of opioid exposure during embryonic development. While neurobehavioral effects of developmental opioid exposure are well-documented, early effects of exposure remain largely unexplored. We investigated the effects of oxycodone and fentanyl exposure on gene expression in zebrafish (Danio rerio) embryos using whole embryo RNA sequencing. Embryos were exposed to environmentally relevant (Oxycodone HCl 10.6pg/mL and Fentanyl Citrate 0.629pg/mL) and therapeutically relevant (Oxycodone HCl 35.14ng/mL and Fentanyl Citrate 3.14ng/mL) from 2 to 24 hours post-fertilization (hpf), followed by another 24hrs of opioid-free development. RNA sequencing at 48hpf revealed dose and drug specific gene expression changes. Lower doses of oxycodone and fentanyl both induced more differentially expressed genes (DEGs) than higher doses, potentially indicative of opioid receptor desensitization occurring at higher concentrations. In total, 892 DEGs were identified across all conditions indicating continued differential gene expression well after cessation of opioid exposure. Gene ontology analysis revealed changes in gene expression relating to extracellular matrix (ECM) organization, cell adhesion, and visual and nervous system formation. Key pathways include axon guidance, synapse formation, and ECM biosynthesis/remodeling all of which have potential implications on neural connectivity and sensory development. These findings demonstrate that developmental exposure to opioids induced persistent transcriptomic changes which may have lasting implications for structural integrity and function in vertebrate nervous systems, providing insights into the molecular mechanisms of opioid-induced alterations during development.
2025-06-04 | GSE292183 | GEO
Project description:Microarrays displaying zebrafish microRNA specific probes were hybridized using RNA samples originating from livers of male zebrafish exposed to 17-beta estradiol (E2) for 0, 24 and 48 hours.
2013-06-02 | E-MEXP-3866 | biostudies-arrayexpress
Project description:Values from samples in this series should be normalised prior to any comparison. Control probes used for normalisation are pre-3, pre-4 and pre-5. Keywords = miRNA Keywords = microRNA Keywords = zebrafish Keywords = Danio rerio Keywords = development Keywords: ordered
2005-07-04 | GSE2625 | GEO
Project description:microRNA-dependent regulation of biomechanical genes establishes tissue stiffness homeostasis
2019-02-19 | PXD011882 | Pride
Project description:This SuperSeries is composed of the following subset Series: GSE38839: MicroRNA expression profiling after short-term exposure to TCDD in zebrafish embryos [agilent and exiqon array data] GSE39808: MicroRNA expression profiling after short-term exposure to TCDD in zebrafish embryos [miRNA-Seq data] Refer to individual Series
2012-08-01 | E-GEOD-39809 | biostudies-arrayexpress
Project description:This study investigates the predictive and prognostic values of inflammatory markers and microRNA in stage IV colorectal cancer. The expression of inflammatory markers and microRNA in plasma will be correlated with tumor location, with dietary patterns and with survival during treatment.
| 2320890 | ecrin-mdr-crc