Transcriptomics

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A new tractable method for generating Human Alveolar Macrophage Like cells in vitro to study lung inflammatory processes and diseases


ABSTRACT: Alveolar macrophages (AM) are unique lung resident myeloid cells and often the first cell type to contact airborne pathogens. The contribution of human AMs (HAM) to pulmonary diseases remains poorly understood due to the difficulty in accessing them from human donors and their rapid phenotypic change during in vitro culture. There remains an unmet need for cost effective methods for generating human cells with a HAM phenotype, particularly important for translational studies and clinical benefits to humans, including analyses of host cell responses to vaccine candidates. We developed cell culture conditions that mimic the lung alveolar environment in humans using lung lipids, that is, Infasurf (calfactant, natural bovine surfactant) and lung-associated cytokines (granulocyte macrophage colony–stimulating factor, transforming growth factor-β, and interleukin 10) that facilitate the conversion of blood-obtained monocytes to an AM-like (AML) phenotype and function in tissue culture. Similar to HAM, AML cells are particularly susceptible to both Mycobacterium tuberculosis and severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infections. This study reveals the importance of alveolar space components in the development and maintenance of HAM phenotype and function and provides a readily accessible model to study HAM in infectious and inflammatory disease processes, as well as therapies and vaccines.

ORGANISM(S): Homo sapiens

PROVIDER: GSE188945 | GEO | 2023/05/08

REPOSITORIES: GEO

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