Dataset Information


Gene expression changes in the human diaphragm following cardiothoracic surgery

ABSTRACT: It is unknown how soon the diaphragm begins to start the process of atrophy following the start of MV. We hypothesized that genes responsible for maintaining diaphragmatic contractile function, stress response, energy transduction would be altered over the course of a 5 hour cardiothoracic surgery. Overall design: Two diaphragm biopsies were obtained from 5 male patients (~67 +/- 11 years) undergoing cardiothoracic surgery. The first biopsy was obtained as soon as the diaphragm was exposed; the second biopsy was obtained as late in the surgery as possible (4.9 +/- 1.8 hours). We profiled mRNA from the 5 pairs of muscle biopsies with a microarray (Affymetrix Human Genome U133 Plus 2.0).

INSTRUMENT(S): [HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array

SUBMITTER: M Cecilia Lopez  

PROVIDER: GSE19533 | GEO | 2012-01-04



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Gene expression changes in the human diaphragm after cardiothoracic surgery.

Huang Tseng-Tien TT   Deoghare Harsha V HV   Smith Barbara K BK   Beaver Thomas M TM   Baker Henry V HV   Mehinto Alvine C AC   Martin A Daniel AD  

The Journal of thoracic and cardiovascular surgery 20110403 5

OBJECTIVE: We examined the effects of cardiothoracic surgery, including cardiopulmonary bypass and controlled mechanical ventilation, on messenger RNA gene expression in human diaphragm. We hypothesized that genes responsible for stress response, redox regulation, protein turnover, energy metabolism, and contractile function would be altered by cardiothoracic surgery. METHODS: Paired diaphragm biopsy samples were obtained from 5 male patients (67 ± 11 years) during cardiothoracic surgery, the fi  ...[more]

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