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Proteome of Young and Old Murine Diaphragm and Associaated Changes in Contractile Properties

ABSTRACT: Proteomic analysis of young and old murine diaphragm and associated changed in contractile aproperties. Redox sensitive Cysteine residues from both adult and old diaphragm tissues examined.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Diaphragm

SUBMITTER: Brian McDonagh

LAB HEAD: Brian McDonagh

PROVIDER: PXD005014 | Pride | 2018-02-12

REPOSITORIES: Pride

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Advanced aging causes diaphragm functional abnormalities, global proteome remodeling, and loss of mitochondrial cysteine redox flexibility in mice.

Kelley Rachel C RC McDonagh Brian B Ferreira Leonardo F LF

Experimental gerontology 20171228

<h4>Aim</h4>Inspiratory muscle (diaphragm) function declines with age, contributing to exercise intolerance and impaired airway clearance. Studies of diaphragm dysfunction in rodents have focused on moderate aging (~24months); thus, the impact of advanced age on the diaphragm and potential mechanisms of dysfunction are less clear. Therefore, we aimed to define the effects of advanced age on the mechanics, morphology, and global and redox proteome of the diaphragm.<h4>Methods</h4>We studied diaph ...[more]

PMID: 29289553

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