Dataset Information


Osmotic stress in Listeria monocytogenes serotype 4b strain F2365 grown in BHI medium and BHI medium with 1.2% NaCl

ABSTRACT: Gene expression profiles of L. monocytogenes strain F2365 to osmotic stress was examined. Based on a log2 scale, four and 24 genes were upregulated and downregulated by the stress, respectively.The expression level of ATP-binding cassette superfamily and ribosomal protein L2 genes were increased, whereas genes associated with PTS system, its metabolic enzymes, pathogenesis, and hypothetical protein were downregulated. Overall design: To examine the gene expression profiles of L. monocytogenes grown in the medium added with or without 1.2% NaCl , L. monocytogenes strains 4b F2365 aminosilane-coated cDNA microarray slide was used. Cy3 and Cy5 dyes were used for cDNA labelling. Data were obtained from three biological and four technical replicates (n = 12). Each biological replicate included a flip dye assay.

INSTRUMENT(S): JCVI PFGRC Listeria monocytogenes 21K vt3a array designed primarily based on strain 4b F2365

ORGANISM(S): Listeria monocytogenes  

SUBMITTER: Chinling Wang  

PROVIDER: GSE19570 | GEO | 2010-01-15



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