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Role of maternal Wnt11b in cortical rotation and axis formation

ABSTRACT: Asymmetric signalling centres in the early embryo are essential for axis formation in vertebrates. These regions, namely the dorsal morula, yolk syncytial layer, and distal hypoblast/anterior visceral endoderm (in amphibians, teleosts and mammals, respectively), require the localised stabilisation of nuclear Beta-catenin (Ctnnb1), implying that localised Wnt/Beta-catenin signalling activity is critical in their establishment. However, it is becoming increasingly apparent that the stabilisation of Beta-catenin in this context may be initiated independently of secreted Wnt growth factor activity. In Xenopus, dorsal Beta-catenin stabilisation is initiated by a requisite microtubule-mediated symmetry-breaking event in the fertilised egg: “cortical rotation”. Vegetally-localised wnt11b mRNA has been implicated upstream of Beta-catenin in this context, as has the dorsal enrichment of Wnt ligand-independent activators of Beta-catenin, but the extent that each of these processes contribute to axis formation in this paradigm remains unclear. Here we describe a maternal effect mutation in Xenopus laevis wnt11b.L, generated by CRISPR mutagenesis. We demonstrate a maternal requirement for timely and complete gastrulation morphogenesis and a zygotic requirement for proper left-right asymmetry. We also show that a subset of maternal wnt11b mutants have axis and dorsal gene expression defects, and we find that microtubule assembly and cortical rotation are reduced in wnt11b mutant eggs, leading to less organised and directed vegetal microtubule arrays. mRNA sequencing was used to determine the extent of dorsal and other gene dysregulation during gastrulation.

ORGANISM(S): Xenopus laevis

PROVIDER: GSE195806 | GEO | 2022/05/02

REPOSITORIES: GEO

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Spemann organizer transcriptome induction by early β-Catenin, Wnt, Nodal and Siamois signals in Xenopus laevis

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2017-03-15 | GSE93195 | GEO

Foxh1/nodal defines context-specific direct maternal Wnt/Beta-catenin target gene regulation in early development

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2020-06-21 | E-MTAB-8555 | biostudies-arrayexpress

Leeuwen2007 - Elucidating the interactions between the adhesive and transcriptional functions of beta-catenin in normal and cancerous cells

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2020-01-09 | MODEL2001090001 | BioModels

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2021-11-17 | PXD022724 | Pride

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2019-02-07 | GSE109417 | GEO

The beta-catenin interactome in a vascular smooth muscle cell model

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2018-10-25 | PXD010421 | Pride

Characterisation of the Beta-Catenin cytoplasmic/nuclear interactome in leukaemia cells

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2019-01-11 | PXD009305 | Pride

Activation of Wnt/beta-catenin in Ewing sarcoma cells antagonizes EWS/ETS function and promotes phenotypic transition to more metastatic cell states

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2023-04-19 | PXD038811 | Pride

USP7 is a tumor-specific WNT activator for APC-mutated colorectal cancer by mediating B-catenin deu

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2017-11-09 | PXD007947 | Pride

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