Project description:To analyze gene expression differences between WT proximal tubule cells and cells in which, by siRNA transfection, we blunted the expression of GLUT2 gene to mimic Fanconi Bickel Syndrome
Project description:We observed a marked increase in efficiency of iPS cell generation when p53 is deleted. Experiment Overall Design: Gene expression patterns were compared between p53 wt MEF and p53-null MEF cells. Experiment Overall Design: Gene expression patterns were also compared between ES cells and MEF.
Project description:Tumor infiltrated treg cells are considered as a crucial in the cancer immunotherapy. We used H3K9me2 CUT&RUN to analyze the differences between WT and jmjd1cTreg cko mice and clarify the important function of jmjd1c in tumor treg. We use ATACseq to analyze the difference between tumor treg and spleen treg and characterize the epigenetic characters in tumor treg.
Project description:To analyze gene expression differences between cells silenced for TFEB and TFE3 vs. Controls, under growing conditions (GC) vs. Fasting conditions (Fast)
Project description:We observed increased severity of immune related adverse events (irAEs) to anti-PD-1 therapy as well as enhanced T cell effector function in miR-146a deficient mice compared to wildtype (WT) mice. The aim of this experiment was to analyze differences in gene expression between WT and miR-146a deficient T cells at the single cell level.
Project description:We aim to determine the physiological impact of inactivating E2F in muscles and in adipose cells in vivo. To uncover the mechanism of action of E2F in each tissue we investigated the similarities between E2F-deficient tissues by comparing the proteomic profiles between muscles and fat cells. Our findings revealed that E2F is required in fat cells to promote the storage of fat and the synthesis of trehalose, which was restored by feeding animals in high sugar diet.
Multiplexed quantitative proteomics was performed using TMT10-plex reagents on an Orbitrap Fusion mass spectrometer using the SPS-MS3 method.
Two sample sets - fatbody samples and samples of thoracic muscles - were analyzed using two TMT sets. Twelve high pH reversed-phase chromatography fractions were analyzed per sample set.
The samples in each set were TMT-labeled as follows:
Fatbody samples:
WT FB MS1; 126
DP FB MS 1; 127n
WT FB MS2; 127c
DP FB MS 2; 128n
WT FB MS3; 128c
DP FB MS 3; 129n
Thoracic muscles samples:
Mef>Ci 1; 126
Mef>Dpi 1; 127n
Mef>Ci 2; 127c
Mef>Dpi 2; 128n
Mef>Ci 3; 128c
Mef>Dpi 3; 129n
Mef>Ci 4; 129c
Mef>Dpi 4; 130n
Project description:In this study, we employed massively parallel sequencing technology to identify miRNAs expressed in Zmpste24 WT MEF and Zmpste24-/- MEF. With data from 19.5 ×106 reads from WT MEFs and 16.5 × 106 reads from Zmpste24-/- MEFs, we discovered a total of 306 known miRNAs expressed in MEFs with a wide dynamic range of read counts ranging from 10 to over 1 million. A total of 8 miRNAs were found to be significantly down-regulated, with only 2 miRNAs upregulated, in Zmpste24-/- MEFs as compared to WT MEFs.
Project description:To analyse gene expression differences between HeLa tranfected cells with mutant COL1 (COL1G610C) compared to their relative scramble controls
Project description:Tumor infiltrated treg cells are considered to be crucial in the cancer immunotherapy. We used single cell RNA sequencing (scRNA-seq) to analyze the differences between WT and jmjd1cTreg cko mice and clarify the important function of jmjd1c.
