Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Gene expression (GeneChip) data from scramble- and CRMP2A siRNA-treated A549 cells.

ABSTRACT: We sought to assess how temporary loss of the microtubule-associated protein CRMP2A could affect global gene expression in the A549 lung cancer cells. We used microarrays to identify differentially regulated genes when CRMP2A is temporarily removed from A549 cells using siRNA.

ORGANISM(S): Homo sapiens

PROVIDER: GSE196499 | GEO | 2022/05/01

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Similar Datasets

Gene expression (GeneChip) data from parental (wild-type) and CRMP2A KO (CRISPR) A549 cells.

Project description:We sought to assess how permanent loss of the microtubule-associated protein CRMP2A could affect global gene expression in the A549 lung cancer cells. We used microarrays to identify differentially regulated genes when CRMP2A is permanently removed from A549 cells using CRISPR/Cas9.

2022-05-01 | GSE196494 | GEO

Gene expression (GeneChip) data from scramble- and CRMP2A siRNA-treated A549 cells.

Project description:Gene expression (GeneChip) data from scramble- and CRMP2A siRNA-treated A549 cells.

| PRJNA805214 | ENA

Comparison of gene expression profiles of A549 cell treated with NRF2 siRNA and control siRNA

Project description:To examine the role of NRF2 in accelerating cell proliferation and to identify the target genes responsible for this function, transcriptome analysis was performed using A549 cells, in which NRF2 is constitutively activated. NRF2 was knocked down by siRNA against NRF2, and the gene expression profile was compared with that of A549 cells treated with control siRNA. To exclude off-target effects, three different siRNAs against NRF2 was independently applied. NRF2 siRNA or control siRNA was transfected into A549 cells. Cells were harvested 24 hours after transfection, and total RNA was purified.

2011-04-01 | E-GEOD-28230 | biostudies-arrayexpress

A549 cells and MSR-A549 cells

Project description:A549 cells and MSR-A549 cells

| PRJNA379619 | ENA

Comparison of gene expression profiles of A549 cell treated with NRF2 siRNA and control siRNA

2011-04-01 | GSE28230 | GEO

Analysis of Nkx2-1-regulated gene expression in A549 lung carcinoma cells

Project description:A transcription factor Nkx2-1 (also known as TTF-1) regulates the expression of different sets of genes. Gene expression analysis was performed using mRNAs from Nkx2-1-induced A549 cells compared to that from the control A549 cells. We used microarrays to detail the global program of gene expression controlled by Nkx2-1 and identified distinct classes of up-regulated and down-regulated genes. mRNAs were isolated from control and Nkx2-1-induced A549 cells and hybridized on Affymetrix microarrays. We sought to identify genes influenced by Nkx2-1.

2012-09-05 | E-GEOD-40584 | biostudies-arrayexpress

Expression data from KEAP1 overexpression and NRF2 siRNA knockdown of A549 NSCLC cell

Project description:KEAP1 overexpressed and NRF2 siRNA knockdown A549 NSCLC cells were used to identify downstream genes of NRF2 pathway separately and by combinatorial analysis. We used triplicate microarrays of transfected A549 cells with mKeap1-GFP for overexpression, siRNAs targeting NRF2 for knockdown and siGFP as control respectively. As a result, we identified several genes which are involved in cancer metabolic functions in these cells. We used microarrays to identify the gene downregulated in both KEAP1 overexpressed and NRF2 siRNA knockdown A549 NSCLC cells and found a subset of downregulated genes which are involved in metabolic functions.

2017-08-02 | GSE94393 | GEO

siRNA profiling of human A549 lung cancer cell line following siRNA mediated knock-down of ALDH1A1 and ALDH3A1

Project description:Aldehyde dehydrogenase isozymes ALDH1A1 and ALDH3A1 are highly expressed in non small cell cell lung cancer. Neither the mechanism nor the biological significance for such over expression have been studied. We used microarrays to analyze changes in A549 lung cancer cell line in which ALDH activity was reduced using lentiviral mediated expression of siRNA against both isozymes (Lenti 1+3) Experiment Overall Design: A549 lung cancer cell lines were transduced with lentiviral vectors containing specific siRNA sequences against ALDH1A1, ALDH3A1, both vectors (Lenti 1+3 cells), and against the green flourescent protein (GFP) gene (GFP cells, used as control).

2008-11-13 | E-GEOD-8045 | biostudies-arrayexpress

Genome-wide transcriptional profiling of Legionella Pneumophila-infected A549 cells transfected with siRNA against TNFAIP2

Project description:In this study we transfected A549 cells with siRNA against TNFAIP2, infected them with L. pneumophila and performed transcriptional profiling. We found enrichment of genes in pro-inflammatory pathways by Pathway Over-respresentation analysis upon infection. There was no significant change in gene expression that we could attribute specifically to the knockdown of TNFAIP2. Examination of the transcriptional response of A549 cells to Legionella infection with concomitant TNFAIP2 knockdown

2016-04-13 | E-GEOD-80214 | biostudies-arrayexpress

Effect of Influenza virus infection on lncRNA expression in A549 cells

Project description:Long non-coding RNAs (lncRNAs) are a new arm of gene regulatory mechanism as discovered by sequencing techniques and follow-up functional studies. There are only few studies on lncRNAs as related to gene expression regulation and anti-viral activity during influenza virus infection. We sought to identify and characterize lncRNAs involved in influenza virus replication. In the current study, we identified dys-regulated lncRNAs in influenza virus-infected human lung epithelial A549 cells using RNA sequencing in A549 cells.

2018-10-12 | GSE121155 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data