Dataset Information


Identification of miR-125b-2 target genes

ABSTRACT: The goal of this study is to define miR-125b-2 target genes in the hematopoietic system by genetic alteration of miR-125b expression levels. Here we report the identification of miR-125b-2 targets in the hematopoietic system by repressing miR125b in megakaryoblastic leukemia (AMKL) cell lines and overexpressing miR-125b-2 in hematopoietic stem and progenitor cells (CD34+-HSPCs) Overall design: M07 and CMK cells were transfected with Cy3-labelled non-silencing olignucleotides or anti-miR-125b and sorted (two experiments). HSPCs were retrovirally transduced and sorted for GFP positive cells. We generated their expression profiles by microarray.


INSTRUMENT(S): [HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array

SUBMITTER: Jan-Henning Klusmann  

PROVIDER: GSE19680 | GEO | 2009-12-29



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miR-125b-2 is a potential oncomiR on human chromosome 21 in megakaryoblastic leukemia.

Klusmann Jan-Henning JH   Li Zhe Z   Böhmer Katarina K   Maroz Aliaksandra A   Koch Mia Lee ML   Emmrich Stephan S   Godinho Frank J FJ   Orkin Stuart H SH   Reinhardt Dirk D  

Genes & development 20100301 5

Children with trisomy 21/Down syndrome (DS) are at high risk to develop acute megakaryoblastic leukemia (DS-AMKL) and the related transient leukemia (DS-TL). The factors on human chromosome 21 (Hsa21) that confer this predisposing effect, especially in synergy with consistently mutated transcription factor GATA1 (GATA1s), remain poorly understood. Here, we investigated the role of Hsa21-encoded miR-125b-2, a microRNA (miRNA) overexpressed in DS-AMKL/TL, in hematopoiesis and leukemogenesis. We id  ...[more]

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