Transcriptomics,Genomics

Dataset Information

20

Time course of the mRNA expression after bacteriophage ϕYS40 infection in wild-type T. thermophilus HB8 strain


ABSTRACT: We observed the expression profile of the total mRNA of wild-type Thermus thermophilus HB8 strain during infection of bacteriophage ϕYS40. Overall design: Wild-type T. thermophilus HB8 cells were pre-cultured at 70°C for 16 h in 3 ml of TT medium containing 0.8% polypeptone, 0.4% yeast extract, 0.2% NaCl, 0.4 mM CaCl2, and 0.4 mM MgCl2, which was adjusted to pH 7.2 with NaOH. The cells (2 ml) were inoculated into 1 liter of the same medium and then cultivated at 70°C until A600 value being ~0.8 (1.7 108 cells/ml) that corresponds to logarithmic growth phase. Then the FYS40 phage was infected to the medium at multiplicity of infection (m.o.i.) being ~1, and continued cultivation. Cells were collected after 0, 25, 50, 75 and 100 min infection, and then crude RNA was extracted. The expression of each mRNA at each time point was analyzed on a GeneChip as described under Sample Description Sheet of each sample.

INSTRUMENT(S): [TTHB8401a520105F] Riken Thermus thermophilus HB8 4K mRNA custom array, library Rev.2

SUBMITTER: Akeo Shinkai  

PROVIDER: GSE19759 | GEO | 2010-10-15

SECONDARY ACCESSION(S): PRJNA129749

REPOSITORIES: GEO

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Publications

Identification of novel genes regulated by the oxidative stress-responsive transcriptional activator SdrP in Thermus thermophilus HB8.

Agari Yoshihiro Y   Kuramitsu Seiki S   Shinkai Akeo A  

FEMS microbiology letters 20101105 2


The stationary phase-dependent regulatory protein (SdrP) from the extremely thermophilic bacterium, Thermus thermophilus HB8, a CRP/FNR family protein, is a transcription activator, whose expression increases in the stationary phase of growth. SdrP positively regulates the expression of several genes involved in nutrient and energy supply, redox control, and nucleic acid metabolism. We found that sdrP mRNA showed an increased response to various environmental or chemical stresses in the logarith  ...[more]

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