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Drn/drn-l expressing cells vs non-expressing cells-Embryonic patterning in Arabidopsis thaliana

ABSTRACT: adt05-04_drn - drn/drn-l expressing cells vs non-expressing cells - DRN targets - In primordia timing (pt) mutant background DRN::erGFP transgenic cell culture (somatic embryos suitable to regenerate plants) has been protoplasted and protoplasts have been sorted for GFP+ and GFP- cells. Total RNA was extracted and sent to Evry for comparison (samples DRN a,b,c). DRN-L::erGFP transgenic cell culture has been protoplasted and protoplasts have been sorted for GFP+ and GFP- cells (samples DRN-L-2-1 and DRN-L-3-1). Keywords: null

ORGANISM(S): Arabidopsis thaliana

PROVIDER: GSE19799 | GEO | 2010/05/01

SECONDARY ACCESSION(S): PRJNA121827

REPOSITORIES: GEO

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drn/drn-l expressing cells vs non-expressing cells-Embryonic patterning in Arabidopsis thaliana

Project description:adt05-04_drn - drn/drn-l expressing cells vs non-expressing cells - DRN targets - In primordia timing (pt) mutant background DRN::erGFP transgenic cell culture (somatic embryos suitable to regenerate plants) has been protoplasted and protoplasts have been sorted for GFP+ and GFP- cells. Total RNA was extracted and sent to Evry for comparison (samples DRN a,b,c). DRN-L::erGFP transgenic cell culture has been protoplasted and protoplasts have been sorted for GFP+ and GFP- cells (samples DRN-L-2-1 and DRN-L-3-1). Keywords: null 5 dye-swap - CATMA arrays

2010-04-30 | E-GEOD-19799 | biostudies-arrayexpress

viromouv-Analysis of the genic expression modifications of Arabidopsis Companion cells infected by plants viruses

Project description:ra10-02_viromouv - viromouv - transcriptome analysis of Arabidopsis Companion cells after infection by plants viruses - Transgenic plants expressing GFP under the control of a companion cell specific promoter were infected by two different viruses: LMV and TuYV. Companion cell protoplasts from these plants were sorted by FACS and extracted RNA were compared to healthy companion cell protoplasts.

2012-11-09 | GSE42059 | GEO

Targets of ALTERED PHLOEM DEVELOPMENT (APL)

Project description:We profiled transcripts from sorted phloem cells of wild-type and apl mutants to identify the genes regulated by APL in phloem. We profiled transcripts from sorted phloem cells of wild-type and apl mutants to identify the genes regulated by APL in phloem. Three biological replicates for control WT and apl mutants were used in thes study. Root tissue was protoplasted and sorted using fluorescence-activated cell sorting (FACS). Total RNA was extracted from GFP+ protoplasts.

2014-08-25 | E-GEOD-55222 | biostudies-arrayexpress

viromouv-Analysis of the genic expression modifications of Arabidopsis Companion cells infected by plants viruses

2012-11-09 | E-GEOD-42059 | biostudies-arrayexpress

esr1-drn-Transcriptomic study of the transcription factor ESR1/DRN.

Project description:adt06-01_esr1-drn_dexamethasone - esr1-drn - Identification of the target genes of the ESR1/DRN TF using dexamethasone induction - Identification of the target genes of the ESR1/DRN TF using dexamethasone induction Keywords: normal vs transgenic comparison 5 dye-swap - CATMA arrays

2008-09-25 | E-GEOD-12919 | biostudies-arrayexpress

esr1-drn-Transcriptomic study of the transcription factor ESR1/DRN.

2008-09-26 | GSE12919 | GEO

Time course of the Arabidopsis root stem cells

Project description:The goal of this dataset was to transcriptionally profile the Arabidopsis root stem cells over developmental time. To accomplish this, we protoplasted roots expressing PET111:GFP every 8 hours from 4 days to 6 days old. We then collected GFP negative cells from the PET111:GFP line using cell sorting, as these cells represent the stem cell population. RNA was extracted from cells, libraries were prepared, and RNA-seq was performed. FPKM values were calulcated using Cufflinks.

2019-10-15 | GSE131988 | GEO

Expression data from mouse bone marrow cells expressing renin driven expression of green fluorescent protein.

Project description:Local renin antiotensin systems have been identified for many extra-renal sites. Bone marrow has been proposed as one such site, although the nature of the renin-expressing cell type(s) has not been established. Affymetrix microarrays were used to characterize the expression profile of renin-expressing GFP positive cells. Green fluorescent protein positive cells were sorted from whole bone marrow collected from adult transgenic mice. Bone marrow from several mice was collected and pooled on the day of a FACS sort. A portion of this was reserved for RNA extraction while the remainder was sorted. RNA was prepared with Trizol. Bone marrow collection and sorting was performed 3 times so that microarrays could be run in triplicate.

2014-02-17 | E-GEOD-53916 | biostudies-arrayexpress

Arabidopsis thaliana

Project description:drn/drn-l expressing cells vs non-expressing cells-Embryonic patterning in Arabidopsis thaliana

| PRJNA121827 | ENA

Identification of genes characteristic of primary inguinal or epididymal preadipocyte fibroblasts

Project description:Committed preadipocyte fibroblasts were genetically labelled in transgenic mice by expressing GFP under the control of the locus for Zfp423, a gene controlling preadipocyte determination. These mice are herein referred to as Zfp423-GFP mice. The overall goal was to identify genes differentially expressed between adipogenic GFP+ firboblasts and non-adipogenic GFP- fibroblasts from either inguinal or epididymal fat stromal vascular cultures obtained from Zfp423-GFP mice. Adipose stromal vascular cultures were obtained from inguinal or epididymal fat of 6-8 week-old male Zfp423-GFP mice. GFP+ and GFP- fibroblasts were sorted from fat stromal vascular cultures and then returned to culture. Subconfluent cultures of GFP+ and GFP- cells were compared in triplicate for Affymetrix analysis.

2011-12-31 | E-GEOD-34775 | biostudies-arrayexpress

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