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Dual ontogeny of disease-associated microglia and disease inflammatory macrophages in aging and neurodegeneration

ABSTRACT: Mice brain at different age were digested and cell suspension were prepared. Cells were stained using anti-CD45, F4/80, CD11b and Ly6C and sorted using an cell sorter BD ARIA. Macrophages were then prepared in order to perform single cell RNA-Seq

ORGANISM(S): Mus musculus

PROVIDER: GSE198531 | GEO | 2022/08/08

REPOSITORIES: GEO

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CD160+ and CD160- NK cells from human livers

Project description:Purified NK cells from human liver tissues were first enriched by MACS using NK Cell Isolation Kit (Miltenyi Biotec, German) and CD160+/- hepatic NK cells were isolated by FACS Aria cell sorter (BD Biosciences, United States) to attain a purity greater than 95%.

2020-12-31 | GSE109197 | GEO

CD49a+ and CD49a- NK cells from human livers

Project description:Purified NK cells from human liver tissues were first enriched by MACS using NK Cell Isolation Kit (Miltenyi Biotec, German) and CD49a+/- hepatic NK cells were isolated by FACS Aria cell sorter (BD Biosciences, United States) to attain a purity greater than 95%.

2020-07-07 | GSE109198 | GEO

CD160+ NK cells from intratumoral and peritumoral tissues

Project description:Purified NK cells from human intratumoral and peritumoral tissues tissues were first enriched by MACS using NK Cell Isolation Kit (Miltenyi Biotec, German) and CD96+/- hepatic NK cells were isolated by FACS Aria cell sorter (BD Biosciences, United States) to attain a purity greater than 95%.

2020-12-31 | GSE118114 | GEO

Transcription profiling of mouse Gr1- and Gr1+ monocytes

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2011-01-08 | E-MEXP-2545 | biostudies-arrayexpress

Single cell analysis of atherosclerosis baseline and regression macrophages-surgical model

Project description:CD45+ CD11b+ F4/80+ macrophages were isolated using the BD FACS Aria IIu SORP from pre-transplant baseline (Apoe-/- fed 16 weeks westernd diet) and grafted arches from WT recipient mice (regression). Cells were then sorted using the C1 Fluidigm to analyze their transcriptomes at a single cell level.

2017-05-15 | GSE97941 | GEO

CD96+ and CD96- NK cells from human livers

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Single cell RNA-seq of mouse megakaryocytes from wild type and Nf-e2 knock out mice

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Expression data from cardiac CD11c+MHC II+ cells

Project description:We previously reported that a synthetic Nod1 ligand, FK565, induced coronary arteritis in mice similar to Kawasaki disease. However, the molecular mechanisms underlying this site-specific inflammation have remained elusive. In this study, we found that CD11c+MHC class II+ cells accumulated in the heart of FK565-treated mice prior to arteritis development. We used microarray analysis to detail gene expression of CD11c+MHC class II+ cells. To obtain gene expression profile of CD11c+MHC class II+ cells, we isolated these cells from hearts of FK565-treated mice. Briefly, female mice at 8weeks age were administered 500 μg of FK565 subcutaneously at day 0 and day 3. At day6, murine hearts were removed and digested with collagenase. CD11c+MHC II+ cells were sorted as PI–CD45+Ly6G–NK1.1–CD11b+CD11c+MHC II+ using FACS Aria cell sorter (BD Biosciences). Sorted cells were subjected to RNA preparation. Two independent replicates from ten mice were made.

2015-03-31 | E-GEOD-60506 | biostudies-arrayexpress

Gene expression in inflammatory macrophages, tissue resident macrophages and neutrophils

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