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Probiotic-induced differential gene expression during Salmonella challenge in neonatal chicks

ABSTRACT: Salmonella remains an important enteric pathogen of poultry, primarily due to concerns regarding food-borne illness in humans consuming contaminated poultry products. Specific probiotic cultures are efficacious as a treatment for neonatal poultry to prevent enteric infections (competitive exclusion) due to the exquisite susceptibility of young chicks to pathogens in the hatchery and brooding environment. The objective of this experiment was to analyze transcriptional profiles in the ceca of neonatal chicks using the Arizona Gallus gallus 20.7K Oligo Array v1.0, following treatment with a probiotic culture derived from poultry with and without Salmonella enterica subsp. Enteritidis (SE) challenge. Chicks were obtained from a commercial hatchery, and challenged with SE upon arrival at the laboratory. One hour post-challenge, chicks were treated with a probiotic culture (FM-B11). Treatment groups included: Control (no challenge or treatment, vehicle only), SE (challenged only), B11 (treated only) and SE+B11 (challenged and treated). Samples were obtained at 12h and 24h post-treatment. We observed that administration of a Lactobacillus-based probiotic culture to chicks following challenge with SE reduced SE colonization of the cecae and resulted in differential expression of genes in the cecae. Among all four treatment groups, 309 genes were differentially expressed (p<0.05) at 12h, and 352 genes were differentially expressed (p<0.05) at 24h. Keywords: disease state analysis

ORGANISM(S): Gallus gallus

PROVIDER: GSE19887 | GEO | 2010/01/14

SECONDARY ACCESSION(S): PRJNA122027

REPOSITORIES: GEO

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Probiotic-induced differential gene expression during Salmonella challenge in neonatal chicks

Project description:Salmonella remains an important enteric pathogen of poultry, primarily due to concerns regarding food-borne illness in humans consuming contaminated poultry products. Specific probiotic cultures are efficacious as a treatment for neonatal poultry to prevent enteric infections (competitive exclusion) due to the exquisite susceptibility of young chicks to pathogens in the hatchery and brooding environment. The objective of this experiment was to analyze transcriptional profiles in the ceca of neonatal chicks using the Arizona Gallus gallus 20.7K Oligo Array v1.0, following treatment with a probiotic culture derived from poultry with and without Salmonella enterica subsp. Enteritidis (SE) challenge. Chicks were obtained from a commercial hatchery, and challenged with SE upon arrival at the laboratory. One hour post-challenge, chicks were treated with a probiotic culture (FM-B11). Treatment groups included: Control (no challenge or treatment, vehicle only), SE (challenged only), B11 (treated only) and SE+B11 (challenged and treated). Samples were obtained at 12h and 24h post-treatment. We observed that administration of a Lactobacillus-based probiotic culture to chicks following challenge with SE reduced SE colonization of the cecae and resulted in differential expression of genes in the cecae. Among all four treatment groups, 309 genes were differentially expressed (p<0.05) at 12h, and 352 genes were differentially expressed (p<0.05) at 24h. Keywords: disease state analysis Eight experimental groups with 4 replicates each were analyzed. A reference RNA design was used for this microarray. Equal amounts of amplified RNA (aRNA) from all samples were pooled and labelled with the Alexa 647 to create the reference pool. Each individual sample was labelled with Alexa 555. Each slide was hybridized with both the reference pool and one sample.

2010-05-15 | E-GEOD-19887 | biostudies-arrayexpress

Liver-expressed antimicrobial peptide-2 is downregulated in Eimeria maxima-infected chickens

Project description:Avian coccidiosis is a major disease of poultry caused by the intestinal protozoa Eimeria. Aviagen line A and line B birds show differential susceptibility to Eimeria infection, with line B birds exhibiting higher lesion scores and mortality. The objective of this study was to examine differential intestinal gene expression between line A and B chicks in response to a challenge with Eimeria maxima. Following challenge with 1 x 10^4 oocysts/chick, greater than 40% of line A chicks had lesion scores of 0 to 1 (on 0 to 4 scale), similar to controls. In contrast, all line B challenged chicks at this same dose had lesion scores of 2 to 4. Total RNA was extracted from the jejunum of control and challenged chicks from both lines A and B. Microarrays were used for detecting the expression-changed genes which responsed to the Eimeria challenge. Samples included: four control A chicks, two challenged A chicks with a lesion score of 1 (A/LS1), two challenged A chicks with lesion scores of 3 to 4 (A/LS3-4), four control B chicks, two challenged B chicks with lesion scores of 2 (B/LS2-3) and two challenged B chicks with lesion scores of 4 (B/LS4). The DNA microarrays were processed at the Virginia Bioinformatics Institute (Virginia Tech) core facility. The raw array data were normalized using GC-robust multiple array (GC-RMA) normalization. Analysis of variance was used for differentially expressed genes based on Welch ANOVA (P < 0.05) and probe set lists were ordered using the fold change analysis provided by GeneSpring software.

2011-07-01 | E-GEOD-25588 | biostudies-arrayexpress

Liver-expressed antimicrobial peptide-2 is downregulated in Eimeria maxima-infected chickens

2011-07-01 | GSE25588 | GEO

Proteomic Analysis of the effect of Salmonella challenge on Broiler chicken

Project description:Salmonella enteritidis (SE) is a foodborne pathogen that causes high morbidity and mortality rates in poultry. Liquid chromatography tandem mass spectrometry (LC-MS/MS) proteomics was used to study the effects of Salmonella infection on spleen proteome in broiler chicks. Control (CON; n=60) or Salmonella challenged (CON-SE; n=60) broilers were gavaged with sterile Tryptic soy agar broth or 7.46 x 108 colony-forming units (CFU) of SE. Weight gain and feed intake between 1 and 14 d post-hatching was determined. A subset of chicks was euthanized on D3 and D7 of age (n=4/group/day) and spleen was aseptically removed, and used for proteomic analysis. There was no difference in growth performance between CON and CON-SE. Across the 16 spleen samples 2625 proteins were measured of which 360 proteins were DAP between D3 and D7. Proteins decreased in abundance between days mediated cell cycle progression, those increased in abundance function in cytoskeleton and mRNA processing. Salmonella infection influenced the abundance of 216 proteins (FDR <0.05); increasing proteins involved in redox homeostasis, lysosomal activities, and energy production, while decreasing abundance of proteins involved in developmental progression. Although SE infection did not affect growth performance of experimental chicks, the proteomics signatures of spleen suggest infection was metabolically costly, and energy was diverted from normal developmental processes to potentiate disease resistance mechanisms.

2022-07-22 | MSV000089947 | MassIVE

Transcriptome Analysis Revealed Ameliorative Effects of Bacillus Based Probiotic on Immunity and Metabolism under an Experimentally Induced Eimeria tenella Infection in Chicken

Project description:In order to explore the potential therapeutic mechanisms of Bacillus subtilis (B. subtilis) probiotic under Eimeria tenella (E. tenella) infection, we performed Transcriptome analysis. A total of 63 Chinese native yellow chickens were purchased from a commercial hatchery and reared in 9 coccidia-free cages with free access to feed and water for up to 28 days. Upon arrival, all chicks were weighed and randomly divided into one of three experimental groups with 21 birds in each group. The three groups consisted of negative control untreated non-challenged group (NC), positive control untreated challenged group (PC) and B. subtilis-fed challenged group (BS + ET). Challenged and non-challenged groups were housed in two different rooms for avoiding the transmission of disease. Temperature (32 to 34 °C for 1st week and then decreased by 2 °C per week), humidity (60 to 80%) and light (23 h L:1D) of both rooms were maintained during the entire period of experiment. On day 21 of age, the chickens in group PC and BS + ET were challenged with 6 × 10^4 E. tenella sporulated oocysts, whereas the chickens in group NC were gavaged with normal saline to provide same management stress. On day 28 of age (day 7 post-infection), 3 individual chickens per group were euthanized humanely by direct heart puncture and caecum tissue samples of all groups were collected for analyzing the whole transcriptional responses to the exposure of probiotic under an induced E. tenella infection in chickens. Totally, 1902 DEGs were up-regulated and 1589 DEGs were down-regulated in the positive control group versus negative control group. 341 up-regulated and 124 down-regulated DEGs were found in the comparison of BS + ET group versus NC Group, while 784 up-regulated and 493 down-regulated DEGs were found in BS + ET versus PC group.

2021-05-06 | GSE166905 | GEO

An innate fear response in domestic chicks: screening for short copy number variations associated with tonic immobility

Project description:We performed aCGH analysis among domestic chicks (Gallus gallus) with different sensitivity in the tonic immobility (TI) to identify the copy number aberrants within genes or regions responsible for innate fear responses.

2013-03-31 | GSE38434 | GEO

An innate fear response in domestic chicks: screening for short copy number variations associated with tonic immobility

2013-03-31 | E-GEOD-38434 | biostudies-arrayexpress

Gallus gallus

Project description:Probiotic-induced differential gene expression during Salmonella challenge in neonatal chicks

| PRJNA122027 | ENA

F8 broiler by Leghorn and broiler by Fayoumi spleen at day 7 and 8 after challenge with SE

Project description:In the two F8 advanced crosses of broiler by Leghorn and broiler by Fayoumi, birds at day 1 were challenged with Salmonella enteritidis (SE). Spleen were collected at day 7 and 8. SE bacterial load in spleen were measured. Based on the bacterial load, birds were divided into high and low SE load groups. Keywords: Salmonella enteritidis challenge

2006-06-01 | GSE4273 | GEO

SE challenged in 3 genetic chicken lines

Project description:3 genetic chicken lines (Leghorn G-B1, Fayoumi, broiler) were used. chicken were challenged with SE at day 1, spleen and cecum tissues were collected at 2 hours and 16 hours after post challenge

2010-06-11 | E-GEOD-2562 | biostudies-arrayexpress

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