Serum extracellular vesicles miR-1305 and miR-6785-5p serve as biomarkers for disease severity in psoriasis

ABSTRACT: Background: The role of serum extracellular vesicles (EVs) are less known in psoriasis. Objectives: To explore the transcriptomic profile of serum EVs and the potential biomarkers in psoriasis. Methods: EVs were isolated by differential ultracentrifugation and identified by transmission electron microscope. The diameters of EVs were detected using nanoparticle tracking analysis. Serum EVs-keratinocyte interaction was observed through confocal fluorescence microscopy. miRNA microarray and mRNA microarray were performed in serum EVs (n=4) and skin lesions (n=3), respectively. qRT-PCR and fluorescence in situ hybridization were used to detect the expression of miRNAs in serum EVs and skin lesions (n=15). Bioinformatics analysis was performed to predict the potential target genes and functions of miR-1305 and miR-6785-5p. Western blot, CCK-8 and Elisa were used to detect the EVs’ biomarkers, keratinocytes proliferation and cytokines secretion. Results: A total of 16 miRNAs and 1,725 mRNAs were significantly dysregulated in serum EVs and skin lesions, respectively. miR-1305 was downregulated and miR-6785-5p was upregulated both in serum EVs and skin lesions. Serum EVs could be uptaken by keratinocytes. MiR-1305 was downregulated and miR-6785-5p were upregulated in keratinocytes after cocultured with psoriasis serum EVs compared with controls. Psoriasis serum EVs promoted keratinocytes proliferation and the secretion of CCL20 and IL-8. Serum EVs miR-1305 and miR-6785-5p were associated with disease severity. Conclusions: Serum EVs might be involved in the activation of keratinocytes through loaded miRNAs in psoriasis. Serum EVs miR-1305 and miR-6785-5p can be served as biomarkers of psoriasis.

ORGANISM(S): Homo sapiens

PROVIDER: GSE200637 | GEO | 2025/04/11

REPOSITORIES: GEO