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Single cell transcriptomics of human brain epileptic lesions identifies pro-inflammatory immune mechanisms based on interlacing cellular immune networks

ABSTRACT: We analysed 85780 single cells from epileptic brain tissues using Cellular Indexing of Transcriptomes and Epitopes by Sequencing (CITE-seq) to dissect the architecture of the immuno-transcriptome of the diseased epileptic human brain. dissect the architecture of the immuno-transcriptome of the diseased epileptic human brain. Our approach uncovered in all patients, well organized pro-inflammatory microenvironment pivoting on activated, pro-inflammatory microglia in a closely-knit network where resident cells attract and manipulate in a pro-inflammatory fashion infiltrating innate and adaptive immune cells. These findings were corroborated by ligand-receptor interactome analysis, which demonstrated potential mechanisms of infiltration and by evidence of direct interactions between microglia and T cells likely contributing to pathogenesis.

ORGANISM(S): Homo sapiens

PROVIDER: GSE201048 | GEO | 2022/04/20

REPOSITORIES: GEO

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