Transcriptomics

Dataset Information

25

Interleukin-16-knock-out bone-marrow derived macrophages response to Escherichia coli lipopolysaccharide (LPS)


ABSTRACT: We have employed whole genome microarray expression profiling to identify differently expressed genes following stimulation of interleukin-16-knock-out bone-marrow derived macrophages with Escherichia coli lipopolysaccharide (LPS). Macrophages were stimulated with LPS (100 ng/ml) for 6 hours and a signature was identified that distinguished between infected and control samples. Expression of several genes from this signature was quantified in the same RNA samples by real-time PCR, confirming the predicted macrophage response pattern. Overall design: Gene expression in macrophages was measured at 6 hours after stimulation Three independent experiments were performed.

INSTRUMENT(S): Agilent-014868 Whole Mouse Genome Microarray 4x44K G4122F (Feature Number version)

ORGANISM(S): Mus musculus  

SUBMITTER: Benoit Desnues  

PROVIDER: GSE20208 | GEO | 2010-10-08

SECONDARY ACCESSION(S): PRJNA129801

REPOSITORIES: GEO

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