Transcriptomics,Genomics

Dataset Information

43

Expression data from Arabidopsis suspension cells overexpressing VND6 and SND1


ABSTRACT: Xylem consists of three types of cells: vessel cells, also referred to as tracheary elements (TEs), parenchyma cells, and fiber cells. TE differentiation includes two essential processes, programmed cell death (PCD) and secondary cell wall formation. These two processes are tightly coupled. However, little is known about the molecular mechanism of their gene regulation. Here, we show that VASCULAR-RELATED NAC-DOMAIN 6 (VND6), a master regulator of TEs, regulates these processes in a coordinated manner. We first identified specific genes downstream of VND6 by comparing them with those of SECONDARY WALL-ASSOCIATES NAC DOMAIN PROTEIN1 (SND1), a master regulator of xylem fiber cells, with transformed suspension culture cells in microarray experiments. Overall design: We used three types of Arabidopsis cultured cells: wild type cells, estrogen-inducible VND6 transformed cells, estrogen-inducible SND1 cells. To examine the initial molecular events induced by VND6 and SND1, we sampled three types of cells at 0h and 12h after the addition of estrogen with triplicates.

REANALYSED by: GSE118579

INSTRUMENT(S): [ATH1-121501] Affymetrix Arabidopsis ATH1 Genome Array

SUBMITTER: Kyoko Ohashi-Ito  

PROVIDER: GSE20586 | GEO | 2010-10-28

SECONDARY ACCESSION(S): PRJNA125283

REPOSITORIES: GEO

altmetric image

Publications

Arabidopsis VASCULAR-RELATED NAC-DOMAIN6 directly regulates the genes that govern programmed cell death and secondary wall formation during xylem differentiation.

Ohashi-Ito Kyoko K   Oda Yoshihisa Y   Fukuda Hiroo H  

The Plant cell 20101015 10


Xylem consists of three types of cells: tracheary elements (TEs), parenchyma cells, and fiber cells. TE differentiation includes two essential processes, programmed cell death (PCD) and secondary cell wall formation. These two processes are tightly coupled. However, little is known about the molecular mechanisms underlying these processes. Here, we show that VASCULAR-RELATED NAC-DOMAIN6 (VND6), a master regulator of TEs, regulates some of the downstream genes involved in these processes in a coo  ...[more]

Similar Datasets

2011-04-01 | GSE24169 | GEO
2010-04-30 | E-MEXP-1876 | ArrayExpress
| GSE81077 | GEO
2010-11-24 | BIOMD0000000280 | BioModels
2011-03-31 | BIOMD0000000324 | BioModels
| GSE61774 | GEO
2017-07-26 | BIOMD0000000642 | BioModels
2013-04-01 | E-GEOD-44601 | ArrayExpress
2014-08-12 | E-GEOD-55522 | ArrayExpress
2015-01-31 | MTBLS119 | MetaboLights