Transcriptomics

Dataset Information

20

Characterization of the RNA content of chromatin


ABSTRACT: We deep sequenced chromatin-associated RNAs (CARs) from human fibroblast (HF) cells. This resulted in the identification of 141 intronic regions and 74 intergenic regions harbouring CARS. Overall design: We purified CARs from normal HFs by isolating soluble chromatin after MNase treatment, followed by separation of chromatin fragments of different lengths on a sucrose gradient. CARs were converted into double-stranded cDNAs and sequenced using the Illumina Genome Analyzer I.

INSTRUMENT(S): Illumina Genome Analyzer (Homo sapiens)

ORGANISM(S): Homo sapiens  

SUBMITTER: Anders Isaksson  

PROVIDER: GSE21227 | GEO | 2010-04-13

SECONDARY ACCESSION(S): PRJNA126491

REPOSITORIES: GEO

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