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Losing the maternal effect gene Nlrp2 alters the ovulated mouse oocytes transcriptome and impacts histone demethylase KDM1B expression

ABSTRACT: We performed RNA sequencing on pools of oocytes after inducing ovarian stimulation in wild-type and Nlrp2-null female mice. We found 231 differentially expressed genes (DEGs) in Nlrp2-null COCs compared to WT COCs (123 up- and 108 downregulated; adjusted p<0.05). The DEGs we identified were enriched for processes involved in neurogenesis, gland morphogenesis, and protein metabolism and for post-translationally methylated proteins. When we compared our RNA sequencing results to an oocyte-specific reference transcriptome that contains many previously unannotated transcripts, we found 228 DEGs, including genes not identified with the first analysis. Intriguingly, 68% and 56% of DEGs from the first and second analyses, respectively, overlap with oocyte-specific hyper- and hypomethylated domains.

ORGANISM(S): Mus musculus

PROVIDER: GSE213059 | GEO | 2023/01/25

REPOSITORIES: GEO

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Losing the maternal effect gene Nlrp2 alters the ovulated mouse oocytes transcriptome and impacts histone demethylase KDM1B expression

Project description:Losing the maternal effect gene Nlrp2 alters the ovulated mouse oocytes transcriptome and impacts histone demethylase KDM1B expression

| PRJNA878844 | ENA

Gene expression analysis of granulosa cells from Fzd1 WT and KO mice

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