Project description:We used microarray to study the global transcriptomic changes in the livers of SIRT7 KO mice, which develop spontaneous nonalcoholic fatty liver disease.
Project description:To examine whether energy starvation caused by the increase in rRNA transcription affects liver metabolism, we compared the gene expression profiles of WT and NML-KO livers using Affymetrix microarray technology. We analyzed 5 livers of WT mice and 5 livers of NML-KO mice.
Project description:We employed marker-free single-cell RNA-Seq to characterize comprehensive transcriptional profiles of 52 EpCAM+ cells from postnatal day 3.25 mouse fetal livers. These cells show characters of cholangiocytes. Combined with our previous single cell data of E11.5~P2.5 mouse fetal livers, our data depict the dynamic trajectories with transcriptional profiles at single-cell resolution during mouse liver development, and provide insights into the fate decision and transcriptional control of self-renewal, differentiation and maturation of liver stem/progenitor cells.
Project description:The purpose of this study is to identify the differential transcriptome profiles in WT, hepatic Atg5 KO, TSC1 KO, Atg5/TSC1 DKO, Atg5/TSC1/p62 TKO and Atg5/TSC1/Nrf2 TKO mouse livers. Hepatic mRNA from 2-month-old mice from 5 different mouse strains were extracted and performed for Nextseq analysis in quadruplicates.
Project description:Neurotensin(NTS), a gut hormone, is known to impact whole body energy metabolism. Here we investigated the impact of neurotensin on the liver in fed (ad libitum) and fasted (22h) female mice livers from NTS wild type (WT) and knockout (KO) mice. Our goal was to investigate the regulation of energy metabolism pathways in the liver that are regulated by neurotensin, specifically lipid uptake and mitochondrial metabolism.
