Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

A senescent cell population with ZEB1 transcription factor as its main regulator promotes osteoarthritis in cartilage and meniscus [TC28_FAP_DRUGseq]

ABSTRACT: To investigate the role of FAP in chondrocytes, we transfected an overexpression vector containing cDNA for FAP (Sino Biological; HG10464-UT) into TC28a2 cells using Lipofectamine 3000. We then performed DRUG-seq analysis to determine the impact of FAP on global gene expression.

ORGANISM(S): Homo sapiens

PROVIDER: GSE220068 | GEO | 2023/04/19

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Similar Datasets

A senescent cell population with ZEB1 transcription factor as its main regulator promotes osteoarthritis in cartilage and meniscus [TC28_ZEB1_DRUGseq]

Project description:To investigate the role of ZEB1 in chondrocytes, we transfected an overexpression vector containing cDNA for ZEB1 (Sino Biological; HG17705-UT) into TC28a2 cells using Lipofectamine 3000. We then performed DRUG-seq analysis to determine the impact of ZEB1 on global gene expression.

2023-04-19 | GSE220069 | GEO

A senescent cell population with ZEB1 transcription factor as its main regulator promotes osteoarthritis in cartilage and meniscus

Project description:This SuperSeries is composed of the SubSeries listed below.

2023-04-18 | GSE220244 | GEO

A senescent cell population with ZEB1 transcription factor as its main regulator promotes osteoarthritis in cartilage and meniscus [Cartilage_Meniscus_scRNAseq]

Project description:Osteoarthritis (OA) affects all tissues in the knee joint but therapeutic targets have often been selected for their role in cartilage damage and inflammation and largely omitted consideration of mechanisms that are mediating meniscus damage and more importantly there have been insufficient efforts to determine whether pathogenic processes are shared between tissues. Several scRNA-seq analyses have been performed in OA knees but have been largely restricted to the analysis of a single joint tissue of articular cartilage, or meniscus with the exception of one study that analyzed cartilage and synovium. Here, we performed scRNA-seq analyses of healthy and OA-affected human knee cartilage and menisci to interrogate separate and shared mechanisms in cellular homeostasis and OA pathogenesis.

2023-04-19 | GSE220243 | GEO

Krüppel-like factors-4 and -2 are important regulators of joint tissue cells and protect against tissue destruction and inflammation in osteoarthritis [ChIP-seq]

Project description:ChIP-seq with anti-HA tag antibody in TC28a2 cells transfected with HA-KLF4.We performed genome-wide analysis for KLF4-DNA association in TC28a2 human chondrocyte cells, to elucidate regulatory mechanisms of chondrogenic genes by KLF4.

2022-09-11 | GSE183955 | GEO

RNA-Sequencing of EndoC-BH1 cells Overexpressing OAS1, OAS2, or OAS3

Project description:EndoC-BH1 cells were plated in normal media and forward transfected with lipofectamine 3000 (2ug cDNA) of empty, OAS1, OAS2, or OAS3 plasmids from Vector builder

2024-01-01 | GSE244273 | GEO

Krüppel-like factors-4 and -2 are important regulators of joint tissue cells and protect against tissue destruction and inflammation in osteoarthritis [DRUG-seq]

Project description:We performed global gene expression analysis in Ad-KLF4-, Ad-EGFP- and non-transduced TC28a2 human chondrocyte cells, to study genes regulated by KLF4 comprehensively.

2022-09-11 | GSE183954 | GEO

Proteomic Analysis of the Meniscus Cartilage in Osteoarthritis.

Project description: Not available

| S-EPMC8348647 | biostudies-literature

Apelin Alleviates Meniscus Endothelial Cell Apoptosis in Osteoarthritis.

Project description: Not available

| S-EPMC8777459 | biostudies-literature

Loss of function of MALAT1 on primary human OA osteoblast transcriptome

Project description:Cells: Primary human osteoblasts from subchondral bone of osteoarthritis patients (n=3 patients). Treatments: Cells transfected using Lipofectamine 3000 (Qiagen) with either control LNA (NC) or MALAT1 LNA1 or MALAT1 LNA2 for 24 hours. Total RNA extracted by Qiagen RNeasy columns. RIN values >7 via Agilent Bioanalyser. Analysis: RNA subjected to Quantseq 3' RNA sequencing (Lexogen) and sequenced reads mapped to the hg38 reference human genome.

2021-04-28 | GSE167918 | GEO

Transcriptome comparison of meniscus from patients with and without osteoarthritis.

Project description: Not available

| S-EPMC6007850 | biostudies-literature