Project description:To investigate the function of XAF1 in immune response, we established XAF1 knockout cell lines in which XAF1 gene has been knockout by sgRNA. We then performed gene expression profiling analysis using data obtained from RNA-seq of WT or XAF1 knockout HT29 cells with or without VSV infection
Project description:To investigate the function of XAF1 in chromatin openning. Wild-type or XAF1 knockout HT29 cells were infected with VSV for 0 or 3 hours,ATAC-seq was performed. Examination of chromatin openness in Wild-type or XAF1 knockout HT29 cells.
Project description:To investigate the function of XAF1 in chromatin openning. Wild-type or XAF1 knockout HT29 cells were infected with VSV for 0 or 3 hours, ChIP-seq was did with H3K27Ac antibody. Chromatin immunoprecipitation DNA-sequencing (ChIP-seq) for histone modifications H3K27Ac in HT29 cells.
Project description:To investigate the function of ZUFSP in immune response, we established ZUFSP knockout cell lines in which ZUFSP gene has been knockdown by sgRNA. We then performed gene expression profiling analysis using data obtained from RNA-seq of WT or ZUFSP knockdown THP1 cells with or without VSV infection
