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MiRNA Expression in Senescent and Immortalized BJ fibroblasts

ABSTRACT: Limited data exists regarding changes of microRNA (miRNA) expression during senescence in human cells and no reports correlate telomerase expression with regulation of senescence-related miRNAs. We used miRNA microarrays to provide a detailed account of miRNA profiles for early passage and senescent human foreskin (BJ) fibroblasts as well as early and late passage immortalized fibroblasts (BJ-hTERT) that stably express the human telomerase reverse transcriptase subunit hTERT. The revelation that miRNA expression changes with extended passaging in BJ-hTERT cells will contribute to a comprehensive understanding of the connections between telomerase expression, senescence and processes of cellular aging. Overall design: Four samples were assessed for miRNA expression by array: BJ fibroblast early passage, BJ fibroblast senescent, BJ-hTERT early passage, and BJ-hTERT late passage. Total RNA from each sample was arrayed in duplicate and results were compared to expression in early passage BJ fibroblasts. The duplicate sample of early passage BJ cells was not included in the final analysis.

INSTRUMENT(S): Agilent-016436 Human miRNA Microarray 1.0 G4472A (miRNA ID version)

ORGANISM(S): Homo Sapiens

SUBMITTER: Laura Bonifacio 

PROVIDER: GSE22134 | GEO | 2010-06-05

SECONDARY ACCESSION(S): PRJNA127569

REPOSITORIES: GEO

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Publications

MiRNA profile associated with replicative senescence, extended cell culture, and ectopic telomerase expression in human foreskin fibroblasts.

Bonifacio Laura N LN   Jarstfer Michael B MB  

PloS one 20100901 9

Senescence is a highly regulated process that limits cellular replication by enforcing a G1 arrest in response to various stimuli. Replicative senescence occurs in response to telomeric DNA erosion, and telomerase expression can offset replicative senescence leading to immortalization of many human cells. Limited data exists regarding changes of microRNA (miRNA) expression during senescence in human cells and no reports correlate telomerase expression with regulation of senescence-related miRNAs  ...[more]