Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

RNA-seq of Mycobacteriophage Island3 infection of Mycolicibacterium smegmatis mc2155, Mycolicibacterium smegmatis mc2155(Butters), and Mycolicibacterium smegmatis mc2155(Buttersgp57r)

ABSTRACT: RNA-seq of Mycobacteriophage Island3 infection of Mycolicibacterium smegmatis mc2155, Mycolicibacterium smegmatis mc2155(Butters), and Mycolicibacterium smegmatis mc2155(Buttersgp57r) to assess the impact of Butters lysogen and specifically Buttersgp57r on transcript levels of island3 during infection.

ORGANISM(S): Mycolicibacterium smegmatis MC2 155 Mycobacterium phage Island3 Mycobacterium phage Butters

PROVIDER: GSE221406 | GEO | 2022/12/24

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Similar Datasets

RNA-seq characterization of an infection of Mycobacterium smegmatis by a cluster A4 mycobacteriophage

Project description:We present transcriptomic analysis of an infection by a cluster A4 mycobacteriophage of its host, Mycobacterium smegmatis, at six time points (n = 2). Viral transcription is detectable 5 minutes into infection, and viral mRNA makes up the vast majority of all transcription by 30 minutes. Kampy, like many phages, has a genome organized into two arms bearing early- and late-phase genes. We identify two transcriptional start sites (TSS’s) consistent with an early promoter driving expression of half of the genome, along with a second, late-stage promoter that becomes active around 30 minutes after the start of infection.

2015-02-02 | GSE60592 | GEO

RNA-seq characterization of an infection of Mycobacterium smegmatis by a cluster A4 mycobacteriophage

Project description:RNA-seq characterization of an infection of Mycobacterium smegmatis by a cluster A4 mycobacteriophage

| PRJNA258602 | ENA

Bedaquiline-induced transcriptional response of Mycobacterium smegmatis mc2 155

Project description:Transcriptional profiling of M. smegmatis mc2155 grown on Hartmans de Bont (HdB) supplemented with 0.2% glycerol and 0.05% Tween80 challenged with 2 μg/ml Bedaquiline and Dimethyl sulfoxide (DMSO)

2015-04-06 | GSE59871 | GEO

Micobacterium smegmatis LC-MS/MS

Project description:Proteome comparison of two Mycolicibacterium smegmatis strains, mc2155 and the recombinant strain expressing MTS1338, a small non-coding RNA of Mycobacterium tuberculosis. The recombinant strain was obtained by electroporation of MTS1338-expressing plasmid into M. smegmatis mc2155 cells

2021-09-09 | PXD019813 | Pride

Transcriptomic profile of Mycobacteriophage Giles

Project description:The gene expression during early (30min) and late (2.5hrs) infection of Mycobacteriophage Giles was analyzed, as well as in a Giles lysogen. Total RNA was isolated, depleted of rRNA and prepped for sequencing (Illumina) during early and late infection of Giles and a lysogen.

2013-04-23 | E-GEOD-43434 | biostudies-arrayexpress

Transcriptomic profile of Mycobacteriophage Giles

Project description:The gene expression during early (30min) and late (2.5hrs) infection of Mycobacteriophage Giles was analyzed, as well as in a Giles lysogen.

2013-04-23 | GSE43434 | GEO

The AmtR regulon of Mycobacterium smegmatis - Transcription profile of an M. smegmatis amtR mutant (JR258) compared to M. smegmatis mc2155 (wild-type)

Project description:To identify the AmtR regulon of Mycobacterium smegmatis, we created a markerless deletion of the amtR gene in the background of strain M. smegmatis mc2155 (wild-type) and compared the transcription profile of both strains grown in batch culture under aerobic conditions on Hartmans de Bont medium supplemented with glycerol (carbon source) and lysine (sole nitrogen source) using microarray. Cells were harvested in early exponential growth stage.

2016-08-31 | E-MTAB-4857 | biostudies-arrayexpress

Transciptional profile of a M. smegmatis mc2155 ΔsigF strain under standard growth conditions

Project description:Comparing transciptional profile of a M. smegmatis mc2155 ΔsigF strain and wild type (control) in in vitro culture at two time points (exponential phase and stationary phase) for identification of SigF-dependent genes.

2010-03-31 | GSE19145 | GEO

Transcriptome Landscape of Mycobacterium smegmatis

Project description:The non-pathogenic bacterium Mycobacterium smegmatis mc2155 has been widely used as a model organism in mycobacterial research, yet a detailed study about its transcription landscape remains to be established. Here we report the transcriptome, expression profiles and transcriptional structures through growth-phase-dependent RNA sequencing (RNA-seq) as well as other related experiments. We found: (1) 2,139 transcriptional start sites (TSSs) in the genome-wide scale, of which eight samples were randomly selected and further verified by 5'-RACE; (2) 2,233 independent monocistronic or polycistronic mRNAs in the transcriptome within the operon/sub-operon structures which are classified into five groups; (3) 47.50% (1016/2139) genes were transcribed into leaderless mRNAs, with the TSSs of 41.3% (883/2139) mRNAs overlapping with the first base of the annotated start codon. Initial amino acids of MSMEG_4921 and MSMEG_6422 proteins were identified by Edman degradation, indicating the presence of distinctive widespread leaderless features in M. smegmatis mc2155. (4) 150 genes with potentially wrong structural annotation, of which 124 proposed genes have been corrected; (5) eight highly active promoters, with their activities further determined by β-galactosidase assays. These data integrated the transcriptional landscape to genome information of model organism mc2155 and lay a solid foundation for further works in Mycobacterium.

2017-08-29 | GSE103158 | GEO

Mycolicibacterium smegmatis strain:mc2155

Project description:Mycolicibacterium smegmatis strain:mc2155 Genome sequencing

| PRJNA749550 | ENA