Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Single-nucleus RNA-seq of microvessel-enriched vascular cells and corresponding parenchyma from frontal cortex samples

ABSTRACT: Vascular cell types are under-represented in standard single-nucleus RNA-Seq studies of human frozen post-mortem brain tissue. Using a novel microvessel-enrichment procedure allows to obtain highly purified fractions of vascular cell types and their corresponding parenchymal fraction of the same post-mortem brain tissue sample.

ORGANISM(S): Homo sapiens

PROVIDER: GSE222007 | GEO | 2023/01/05

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Shared Molecules

Only show the datasets with similarity scores above: 0.5

Threshold

0.5

Similar Datasets

snRNA-seq of nuclei isolated with microvessel-enrichment procedure applied to frozen post-mortem brain tissue

Project description:Vascular cell types are under-represented in standard single-nucleus RNA-Seq studies of human frozen post-mortem brain tissue. The dataset represents the pilot data obtained with the first version of a microvessel- enrichment protocol to obtain a higher percentage of endothelial cells as the key cellular component of the blood-brain barrier.

2023-03-08 | GSE226606 | GEO

Rat brain microvessel SAGE

Project description:SAGE analysis of genes expressed in rat brain microvessels. Keywords: Novel gene identification Microvessels were pooled from 50 rats, a single SAGE library was constructed from the microvessel RNA

2005-12-02 | E-GEOD-3696 | biostudies-arrayexpress

single-nucleus RNA-seq of microvessel-enriched vascular cells and corresponding parenchyma from frontal cortex samples

Project description:single-nucleus RNA-seq of microvessel-enriched vascular cells and corresponding parenchyma from frontal cortex samples

| PRJNA917304 | ENA

snRNA-seq of nuclei isolated with microvessel-enrichment procedure applied to frozen post-mortem brain tissue

Project description:snRNA-seq of nuclei isolated with microvessel-enrichment procedure applied to frozen post-mortem brain tissue

| PRJNA940797 | ENA

Brain microvessel-specific transcriptome profiling after severe cerebral hypoperfusion in mice

Project description:Little is understood about the underlying cellular and molecular mechanisms related to brain microvessel damage and glial activation after severe cerebral hypoperfusion. Efforts to explore the relationship between neuropathological and gene expression changes support a role for identifying novel molecular pathways by transcriptomic mechanisms.

2024-12-06 | GSE266214 | GEO

Brain microvessel-specific transcriptome profiling after severe cerebral hypoperfusion in mice

Project description:Brain microvessel-specific transcriptome profiling after severe cerebral hypoperfusion in mice

| PRJNA1106380 | ENA

Brain microvessel: Alzheimer disease vs normal

Project description:The enigma that is Alzheimer's disease (AD) continues to present daunting challenges for effective therapeutic intervention. The lack of disease-modifying therapies may, in part, be attributable to the narrow research focus employed to understand this complex disease. Most studies into disease pathogenesis are based on a priori assumptions about the role of AD lesion-associated proteins such as amyloid-? and tau. However, the complex disease processes at work may not be amenable to single-target therapeutic approaches. Genome-wide expression studies provide an unbiased approach for investigating the pathogenesis of complex diseases like AD. A growing literature suggests a role for cerebrovascular contributions to the pathogenesis of AD. The objective of the current study is to examine human brain microvessels isolated from AD patients and controls by microarray analysis. Differentially expressed genes with more than 2-fold change are used for further data analysis. Gene ontology analysis and pathway analysis algorithms within GeneSpringGX are employed to understand the regulatory networks of differentially expressed genes. Twelve matched pairs of AD and control brain microvessel samples are hybridized to Agilent Human 4 × 44 K arrays in replication. We document that more than 2,000 genes are differentially altered in AD microvessels and that a large number of these genes map to pathways associated with immune and inflammatory response, signal transduction, and nervous system development and function categories. These data may help elucidate heretofore unknown molecular alterations in the AD cerebromicrovasculature. Two condition experiment, diseased vs normal, our study included 12 pairs of biological replicates and each pair was repeated with dye swap making total of 24 double channel hybridizations. we excluded 4 samples for not passing QC. So 20 samples were included in the data analysis.

2013-03-29 | E-GEOD-45596 | biostudies-arrayexpress

Brain microvessel: Alzheimer disease vs normal

Project description:The enigma that is Alzheimer's disease (AD) continues to present daunting challenges for effective therapeutic intervention. The lack of disease-modifying therapies may, in part, be attributable to the narrow research focus employed to understand this complex disease. Most studies into disease pathogenesis are based on a priori assumptions about the role of AD lesion-associated proteins such as amyloid-β and tau. However, the complex disease processes at work may not be amenable to single-target therapeutic approaches. Genome-wide expression studies provide an unbiased approach for investigating the pathogenesis of complex diseases like AD. A growing literature suggests a role for cerebrovascular contributions to the pathogenesis of AD. The objective of the current study is to examine human brain microvessels isolated from AD patients and controls by microarray analysis. Differentially expressed genes with more than 2-fold change are used for further data analysis. Gene ontology analysis and pathway analysis algorithms within GeneSpringGX are employed to understand the regulatory networks of differentially expressed genes. Twelve matched pairs of AD and control brain microvessel samples are hybridized to Agilent Human 4 × 44 K arrays in replication. We document that more than 2,000 genes are differentially altered in AD microvessels and that a large number of these genes map to pathways associated with immune and inflammatory response, signal transduction, and nervous system development and function categories. These data may help elucidate heretofore unknown molecular alterations in the AD cerebromicrovasculature.

2013-03-29 | GSE45596 | GEO

Post-mortem gene expression from mouse brain

Project description:Post-mortem gene expression from mouse brain

| PRJNA672107 | ENA

Rattus norvegicus

Project description:Rat brain microvessel SAGE

| PRJNA93843 | ENA