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AtDGCR14L plays an essential role in salt-stress tolerance via regulating pre-mRNA splicing in Arabidopsis

ABSTRACT: Although pre-mRNA alternative splicing has been demonstrated to be a crucial layer of gene expression regulation in response to salt stress, the underlying mechanisms remain elusive. Through integrative studies of the singular ortholog of DGCR14 in Arabidopsis thaliana (AtDGCR14L), we discovered that AtDGCR14L plays an essential role in plant salt stress responses by maintaining the constitutively spliced and active isoforms of important stress- and/or ABA-responsive genes. We also identified the interaction between AtDGCR14L and spliceosome component U1-70k mediated by a highly conserved TWG motif in DGCR14, which is crucial for AtDGCR14L’s functions in salt stress responses. Moreover, we discovered the novel role of SWI3A, whose splicing is dependent on AtDGCR14L, in controlling salt stress tolerance. Collectively, these results revealed the central role of AtDGCR14L in linking plant salt-stress tolerance with pre-mRNA splicing mechanism. This work also provided new insights into the mechanism of 22q11.2 deletion-induced developmental disorders in humans.

ORGANISM(S): Arabidopsis thaliana

PROVIDER: GSE222547 | GEO | 2023/09/06

REPOSITORIES: GEO

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