Transcriptomics,Genomics

Dataset Information

23

Gene expression analysis during heat shock in Drosophila melanogaster 3rd instar hsf4 cn bw larvae


ABSTRACT: Gene expression analysis of Drosophila melanogaster 3rd instar hsf4 cn bw larvae, under heat shock and non-heat shock conditions (room temperature) using Nimblegen arrrays (GPL8443) Overall design: total RNA recovered from heat shocked or non-heat shocked (room temperature) samples in hsf4 larvae, 3 biological replicates for each treatment

INSTRUMENT(S): CDMC Nimblegen Drosophila 17K v1

SUBMITTER: Zak Razak   

PROVIDER: GSE22332 | GEO | 2011-01-05

SECONDARY ACCESSION(S): PRJNA127505

REPOSITORIES: GEO

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Publications

Whole-genome analysis reveals that active heat shock factor binding sites are mostly associated with non-heat shock genes in Drosophila melanogaster.

Gonsalves Sarah E SE   Moses Alan M AM   Razak Zak Z   Robert Francois F   Westwood J Timothy JT  

PloS one 20110114 1


During heat shock (HS) and other stresses, HS gene transcription in eukaryotes is up-regulated by the transcription factor heat shock factor (HSF). While the identities of the major HS genes have been known for more than 30 years, it has been suspected that HSF binds to numerous other genes and potentially regulates their transcription. In this study, we have used a chromatin immunoprecipitation and microarray (ChIP-chip) approach to identify 434 regions in the Drosophila genome that are bound b  ...[more]

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